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Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy
- Publication Year :
- 2013
- Publisher :
- Society of Photo-Optical Instrumentation Engineers, 2013.
-
Abstract
- Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculation in ovo in the chorioallantoic membrane of the developing chick are presented. Imaging is performed with a high-resolution rigid Hopkins lens suitable for endoscopy.
- Subjects :
- Optics and Photonics
Materials science
Time Factors
Light
Research Papers: Imaging
Biomedical Engineering
Image registration
Chick Embryo
Chorioallantoic Membrane
law.invention
Biomaterials
Motion
Optics
law
Distortion
Microscopy
Computer Graphics
Animals
Lighting
Microscopy, Video
business.industry
Microcirculation
Endoscopy
Wollaston prism
Equipment Design
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Lens (optics)
Temporal resolution
Prism
business
Artifacts
Preclinical imaging
Algorithms
Software
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....48658733528eb5fea5e94ebd6c4bab5c