Back to Search
Start Over
Active migration into the subcellular space precedes Campylobacter jejuni invasion of epithelial cells
- Source :
- Cellular Microbiology. :070725190509002
- Publication Year :
- 2007
- Publisher :
- Hindawi Limited, 2007.
-
Abstract
- The bacterial pathogen Campylobacter jejuni invades mucosal cells via largely undefined and rather inefficient (0.01-2 bacteria per cell) mechanisms. Here we report a novel, highly efficient C. jejuni infection pathway resulting in 10-15 intracellular bacteria per cell within 3 h of infection. Electron microscopy, pulse-chase infection assays and time-lapse multiphoton laser confocal microscopy demonstrated that the mechanism involved active and rapid migration of the pathogen into the subcellular space (termed 'subvasion'), followed by bacterial entry ('invasion') at the cell basis. Efficient subvasion was maximal after repeated rounds of selection for the subvasive phenotype. Targeted mutagenesis indicated that the CadF, JlpA or PEB1 adhesins were not required. Dissection of the selected and parental phenotypes by SDS-PAGE yielded comparable capsule polysaccharide and lipooligosaccharide profiles. Proteomics revealed reduced amounts of the chemotaxis protein CheW for the subvasive phenotype. Swarming assays confirmed that the selected phenotype exhibited altered migration behaviour. Introduction of a plasmid carrying chemotaxis genes into the subvasive strain yielded wild-type subvasion levels and migration behaviour. These results indicate that alterations in the bacterial migration machinery enable C. jejuni to actively penetrate the subcellular space and gain access to the cell interior with unprecedented efficiency.
- Subjects :
- DNA, Bacterial
Lipopolysaccharides
Proteome
Molecular Sequence Data
Immunology
Cell
Intracellular Space
Microbiology
Campylobacter jejuni
Cell Line
Bacterial genetics
Bacterial Proteins
Microscopy, Electron, Transmission
Virology
medicine
Humans
Adhesins, Bacterial
Pathogen
Antigens, Bacterial
Microscopy, Confocal
Microscopy, Video
biology
Chemotaxis
Intracellular parasite
Genetic Complementation Test
Polysaccharides, Bacterial
Epithelial Cells
Sequence Analysis, DNA
biology.organism_classification
Phenotype
Bacterial adhesin
Mutagenesis, Insertional
medicine.anatomical_structure
Electrophoresis, Polyacrylamide Gel
Carrier Proteins
Locomotion
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 14625822 and 14625814
- Database :
- OpenAIRE
- Journal :
- Cellular Microbiology
- Accession number :
- edsair.doi.dedup.....4858f69cfb367b557507dea08cd1a625