Back to Search
Start Over
Mycoplasma hyopneumoniae J elicits an antioxidant response and decreases the expression of ciliary genes in infected swine epithelial cells
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-22 (2020), Scientific Reports, Scientific Reports, 2020, 10 (13707), ⟨10.1038/s41598-020-70040-y⟩, Scientific Reports, Nature Publishing Group, 2020, 10 (13707), ⟨10.1038/s41598-020-70040-y⟩
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Mycoplasma hyopneumoniae is the most costly pathogen for swine production. Although several studies have focused on the host-bacterium association, little is known about the changes in gene expression of swine cells upon infection. To improve our understanding of this interaction, we infected swine epithelial NPTr cells with M. hyopneumoniae strain J to identify differentially expressed mRNAs and miRNAs. The levels of 1,268 genes and 170 miRNAs were significantly modified post-infection. Up-regulated mRNAs were enriched in genes related to redox homeostasis and antioxidant defense, known to be regulated by the transcription factor NRF2 in related species. Down-regulated mRNAs were enriched in genes associated with cytoskeleton and ciliary functions. Bioinformatic analyses suggested a correlation between changes in miRNA and mRNA levels, since we detected down-regulation of miRNAs predicted to target antioxidant genes and up-regulation of miRNAs targeting ciliary and cytoskeleton genes. Interestingly, most down-regulated miRNAs were detected in exosome-like vesicles suggesting that M. hyopneumoniae infection induced a modification of the composition of NPTr-released vesicles. Taken together, our data indicate that M. hyopneumoniae elicits an antioxidant response induced by NRF2 in infected cells. In addition, we propose that ciliostasis caused by this pathogen is partially explained by the down-regulation of ciliary genes.
- Subjects :
- 0301 basic medicine
Swine
[SDV]Life Sciences [q-bio]
lcsh:Medicine
Article
Antioxidants
03 medical and health sciences
0302 clinical medicine
Mycoplasma hyopneumoniae
Bacterial Proteins
Gene expression
microRNA
Animals
Cilia
RNA, Messenger
Transcriptomics
lcsh:Science
Gene
Pathogen
Transcription factor
Cells, Cultured
Regulation of gene expression
Multidisciplinary
biology
Gene Expression Profiling
lcsh:R
Bacteriology
Epithelial Cells
Pneumonia of Swine, Mycoplasmal
biology.organism_classification
3. Good health
Cell biology
Gene expression profiling
MicroRNAs
030104 developmental biology
Gene Expression Regulation
miRNAs
lcsh:Q
Pathogens
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
030217 neurology & neurosurgery
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....4854b664938cf252dcb824c2173f52eb