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Deoxyribonuclease 1 reduces pathogenic effects of cigarette smoke exposure in the lung

Authors :
Saleela Ruwanpura
Paul T. King
Kim M. O’Sullivan
Belinda J. Thomas
Lovisa Dousha
Judy M. Callaghan
Stavros Selemidis
Roleen Sharma
Gavin De Carle Brooks
Michael Farmer
Michaela Finsterbusch
Gary P. Anderson
Barton R Jennings
Steven Lim
Philip G. Bardin
Stephen R. Holdsworth
Source :
Scientific Reports, Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
Publication Year :
2017
Publisher :
Springer Science and Business Media LLC, 2017.

Abstract

Our aim was to investigate if deoxyribonuclease (DNase) 1 is a potential therapeutic agent to reduce pathogenic effects of cigarette smoke exposure in the lung. Cigarette smoke causes protease imbalance with excess production of proteases, which is a key process in the pathogenesis of emphysema. The mechanisms responsible for this effect are not well-defined. Our studies demonstrate both in vitro and in vivo that cigarette smoke significantly increases the expression of neutrophil and macrophage extracellular traps with coexpression of the pathogenic proteases, neutrophil elastase and matrix metalloproteinases 9 and 12. This response to cigarette smoke was significantly reduced by the addition of DNase 1, which also significantly decreased macrophage numbers and lung proteolysis. DNase 1, a treatment currently in clinical use, can diminish the pathogenic effects of cigarette smoke.

Details

ISSN :
20452322
Volume :
7
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....4853588de49460d7e8e01050b6da1d4f
Full Text :
https://doi.org/10.1038/s41598-017-12474-5