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Structure and expression of bombyxin E1 gene: a novel family gene that encodes bombyxin-IV, an insect insulin-related neurosecretory peptide
- Source :
- Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 117(3)
- Publication Year :
- 1997
-
Abstract
- A bombyxin gene encoding precursor molecule for bombyxin-IV, one of the insulin-related neurosecretory peptide of the silkmoth Bombyx mori, has been cloned and characterized. The nucleotide sequence of this gene and its deduced amino acid sequence deviate moderately from those characterized previously for the family A, B, C and D bombyxin genes. The gene encoding the bombyxin-IV precursor was therefore defined into a novel family E and designated as gene E1. The bombyxin E1 transcript in Bombyx brain was shown to locate in four pairs of medial neurosecretory cells, which also produce other bombyxin family mRNAs, and the amount of the E1 transcript did not change markedly during the fifth larval instar. Genomic Southern hybridization indicated that the Bombyx haploid genome contained a single copy of the bombyxin family E gene.
- Subjects :
- Physiology
Molecular Sequence Data
Nerve Tissue Proteins
In situ hybridization
Haploidy
Biochemistry
Bombyx mori
Animals
Amino Acid Sequence
RNA, Messenger
Molecular Biology
Gene
Peptide sequence
In Situ Hybridization
Bombyx
Southern blot
Genetics
Brain Chemistry
biology
Base Sequence
fungi
Neuropeptides
Nucleic acid sequence
RNA
biology.organism_classification
Molecular biology
Insect Hormones
Insect Proteins
Subjects
Details
- ISSN :
- 10964959
- Volume :
- 117
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology
- Accession number :
- edsair.doi.dedup.....483df47344a5608e3e25437f4389f9d4