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Molecular identification of four cryptic species of Mastomys (Rodentia, Murinae)

Authors :
Karine Mouline
Jean-François Cosson
Anne Loiseau
Jean-Marc Duplantier
Maxime Galan
Carine Brouat
Laurent Granjon
Emilie Lecompte
Muséum national d'Histoire naturelle (MNHN)
Service de Systématique Moléculaire (SSM)
Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)
Institut für Virologie
Philipps University
Centre de Biologie pour la Gestion des Populations (UMR CBGP)
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Institut de recherche pour le développement (IRD [Sénégal])
Laboratoire de Mammalogie
Musée royal de l'Afrique centrale
Source :
Biochemical Systematics and Ecology, Biochemical Systematics and Ecology, Elsevier, 2005, 33 (7), pp.681-689. ⟨10.1016/j.bse.2004.12.015⟩
Publication Year :
2005
Publisher :
HAL CCSD, 2005.

Abstract

Corresponding author. cosson@supagro.inra.fr; International audience; Multimammate rats (genus Mastomys) are abundant in many regions throughout sub-Saharan Africa, and are of high economical and sanitary importance as agricultural pests as well as reservoir/vectors of human diseases. In pest management and in epidemiological studies, unequivocal species identification of individuals collected in the field is crucial. However, the discrimination among most of the Mastomys species is often difficult, if not impossible, on the basis of external characters. Karyology provides unambiguous specific assignations, but is not suitable for population studies involving large numbers of individuals because it requires fresh material and/or quick transfer from the field to the laboratory. The purpose of this study was to search for molecular markers allowing a clear discrimination of field collected individuals on the basis of ethanol-preserved samples. Using sequences of the cytochrome b region of mitochondrial DNA, two molecular tests based on species-specific primers (test 1) and restriction sites generating species-specific profiles (test 2), were designed and evaluated for species identification on a large number of karyotypically or electrophoretically unambiguously determined individuals. The tests clearly discriminate the four most widespread species. They are easy to perform on a small piece of car or tail taken from live animals, and can probably be adapted to identify museum specimens

Details

Language :
English
ISSN :
03051978
Database :
OpenAIRE
Journal :
Biochemical Systematics and Ecology, Biochemical Systematics and Ecology, Elsevier, 2005, 33 (7), pp.681-689. ⟨10.1016/j.bse.2004.12.015⟩
Accession number :
edsair.doi.dedup.....483ddc6495fb2618d8f1d67190a9d5fa
Full Text :
https://doi.org/10.1016/j.bse.2004.12.015⟩