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Anticancer Activity of Novel Plant Extracts and Compounds from Adenosma bracteosum (Bonati) in Human Lung and Liver Cancer Cells
- Source :
- Molecules, Volume 25, Issue 12, Molecules, Vol 25, Iss 2912, p 2912 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Cancer is the second leading cause of death globally, and despite the advances in drug development, it is still necessary to develop new plant-derived medicines. Compared with using conventional chemical drugs to decrease the side effects induced by chemotherapy, natural herbal medicines have many advantages. The present study aimed to discover the potential cytotoxicity of ethanol extract and its derived fractions (chloroform, ethyl acetate, butanol, and aqueous) of Adenosma bracteosum Bonati. (A. bracteosum) on human large cell lung carcinoma (NCI-H460) and hepatocellular carcinoma (HepG2). Among these fractions, the chloroform showed significant activity in the inhibition of proliferation of both cancerous cells because of the presence of bioactive compounds including xanthomicrol, 5,4&rsquo<br />dihydroxy-6,7,8,3&rsquo<br />tetramethoxyflavone, and ursolic acid which were clearly revealed by nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR, Heteronuclear Multiple Bond Coherence, and Heteronuclear Single Quantum Coherence Spectroscopy) analyses. According to the radical scavenging capacity, the 5,4&rsquo<br />tetramethoxyflavone compound (AB2) exhibited the highest anticancer activity on both NCI-H460 and HepG2 with IC50 values of 4.57 &plusmn<br />0.32 and 5.67 &plusmn<br />0.09 &micro<br />g/mL respectively, followed by the ursolic acid with the lower percent inhibition at 13.05 &plusmn<br />0.55 and 10.00 &plusmn<br />0.16 &micro<br />g/mL, respectively (p &lt<br />0.05). Remarkably, the AB2 compound induced to significant increase in the production of reactive oxygen species accompanied by attenuation of mitochondrial membrane potential, thus inducing the activation of caspase-3 activity in both human lung and liver cancer cells. These results suggest that A. bracteosum is a promising source of useful natural products and AB2 offers opportunities to develop the novel anticancer drugs.
- Subjects :
- caspase-3
Ethyl acetate
Pharmaceutical Science
Pharmacology
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
lcsh:Organic chemistry
Ursolic acid
Drug Discovery
medicine
Adenosma bracteosum
Physical and Theoretical Chemistry
Cytotoxicity
extract
anti-cancer
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
Chloroform
Ethanol
Organic Chemistry
cell line
medicine.disease
isolated compounds
chemistry
Chemistry (miscellaneous)
Cell culture
030220 oncology & carcinogenesis
Large-cell lung carcinoma
Molecular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....483d6fe9d8fa09144e11bc89290ccf3d
- Full Text :
- https://doi.org/10.3390/molecules25122912