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A checkpoint in B-lymphopoiesis related to Notch resistance
- Source :
- Biochemical and Biophysical Research Communications. 417:141-146
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- While murine B- and T-lymphopoiesis require overlapping molecules, they occur in separate organs: the bone marrow (BM) and the thymus, respectively. The BM microenvironment is incapable of supporting T-lymphopoiesis because of insufficient interactions of Notch1 with delta-like ligand (Dll). Notch1/Dll interactions also play a role in the suppression of B-lymphopoiesis in the thymus. However, it is still unclear whether the Notch1/Dll interaction alone explains why the thymus does not support B-lymphopoiesis. In this study, we compared the precursor population colonizing the thymus with that in the BM by culturing them on stromal cells expressing abundant Dll1. We demonstrated that Flt3(+) Il7r(+) B220(+) Cd19(+) BM cells gave rise to B cells under this condition. We defined them as resistant to Dll1. In the thymus, Dll1-resistant cells were undetectable. This suggested that the absence of Dll1-resistant cells might explain the absence of B-lymphopoiesis in the thymus.
- Subjects :
- medicine.medical_specialty
Stromal cell
Antigens, CD19
Population
Biophysics
Mice, Transgenic
Thymus Gland
Biochemistry
CD19
Mice
hemic and lymphatic diseases
Internal medicine
medicine
Animals
Lymphopoiesis
Receptor, Notch1
education
Interleukin-7 receptor
Molecular Biology
Cells, Cultured
B-Lymphocytes
education.field_of_study
Receptors, Interleukin-7
biology
Intracellular Signaling Peptides and Proteins
Membrane Proteins
Cell Biology
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
Endocrinology
fms-Like Tyrosine Kinase 3
Membrane protein
Fms-Like Tyrosine Kinase 3
biology.protein
Leukocyte Common Antigens
Bone marrow
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 417
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....483b1b948d9be1fe45b5c496e2a6a2ee