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Factors influencing environmental sampling recovery of healthcare pathogens from non-porous surfaces with cellulose sponges
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0261588 (2022), PLoS ONE
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Results from sampling healthcare surfaces for pathogens are difficult to interpret without understanding the factors that influence pathogen detection. We investigated the recovery of four healthcare-associated pathogens from three common surface materials, and how a body fluid simulant (artificial test soil, ATS), deposition method, and contamination levels influence the percent of organisms recovered (%R). Known quantities of carbapenemase-producing KPC+ Klebsiella pneumoniae (KPC), Acinetobacter baumannii, vancomycin-resistant Enterococcus faecalis, and Clostridioides difficile spores (CD) were suspended in Butterfield’s buffer or ATS, deposited on 323cm2 steel, plastic, and laminate surfaces, allowed to dry 1h, then sampled with a cellulose sponge wipe. Bacteria were eluted, cultured, CFU counted and %R determined relative to the inoculum. The %R varied by organism, from 1 log10 within the 60 min drying time. For all organisms, the %R was significantly greater if suspended in ATS than if suspended in Butterfield’s buffer (p
- Subjects :
- Acinetobacter baumannii
Physiology
Social Sciences
Pathology and Laboratory Medicine
Microbial Physiology
Medicine and Health Sciences
Psychology
Bacterial Physiology
Materials
Multidisciplinary
Organic Compounds
Body Fluids
Bacterial Pathogens
Klebsiella pneumoniae
Chemistry
Blood
Medical Microbiology
Physical Sciences
Medicine
Anatomy
Pathogens
Plastics
Research Article
Surface Properties
Clostridium Difficile
Science
Materials Science
Enterococcus Faecalis
Microbiology
Specimen Handling
Vancomycin-Resistant Enterococci
Humans
Bacterial Spores
Cellulose
Microbial Pathogens
Behavior
Bacteria
Clostridioides difficile
Organic Chemistry
Gut Bacteria
Organisms
Chemical Compounds
Biology and Life Sciences
Bacteriology
Bandages
Health Care
Steel
Health Care Facilities
Sedentary Behavior
Enterococcus
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....48283be3d96273f2c7bca312870d1f22