Back to Search
Start Over
Modulation of hippocampal neuronal activity by So-ochim-tang-gamibang in mice subjected to chronic restraint stress
- Source :
- BMC Complementary and Alternative Medicine, BMC Complementary and Alternative Medicine, Vol 17, Iss 1, Pp 1-10 (2017)
- Publication Year :
- 2017
- Publisher :
- BioMed Central, 2017.
-
Abstract
- Background So-ochim-tang-gamibang (SOCG) is a decoction formula which has been used to improve mental activity in traditional Korean medicine. The present study was performed to evaluate whether the treatment of SOCG was involved in activating hippocampal neurons in mice which were subjected to chronic restraint stress (CRS). Methods Mice were subjected to CRS for 2 weeks to induce depressive-like behaviors. SOCG was orally administered for the same period. mRNA expression in the hippocampus was analyzed by RT-PCR. Levels of serotonin receptor 5-HT1AR in the hippocampus were determined by western blotting and by immunofluorescence staining in coronal brain sections. Cultured neurons were prepared from the dorsal root ganglia (DRG) in mice to examine the effects of CRS and SOCG treatment on neurite outgrowth. Depressive-like behaviors of experimental animals were measured by open field test (OFT) and forced swimming test (FST). Results mRNA levels of serotonin 1A and 1B receptors (5-HT1AR and 5-HT1BR) were decreased in the hippocampus of CRS animals and increased by SOCG treatment. Signals of 5-HT1AR protein in CA3 pyramidal cells were decreased by CRS but elevated back to levels in control animals after SOCG treatment. Phospho-Erk1/2 protein in CA3 cells showed similar pattern of changes as in 5-HT1AR, suggesting coordinated regulation after SOCG treatment in CRS animals. Axonal growth-associated protein GAP-43 levels were also decreased by CRS and then increased by SOCG treatment. In vivo administration of SOCG improved neurite outgrowth of primary DRG neurons from CRS animals and also increased 5-HT1AR protein signals. Behavioral tests of open field and forced swimming showed that immobility time periods were significantly decreased by SOCG treatment. Conclusions Our data suggest that SOCG treatment may increase synaptic responsiveness to serotonergic neuronal inputs by upregulating 5-HT1AR in the hippocampal neurons.
- Subjects :
- 0301 basic medicine
Male
Restraint, Physical
medicine.medical_specialty
Neurite
Hippocampus
Hippocampal formation
So-ochim-tang-gamibang
Serotonergic
Open field
03 medical and health sciences
Chronic restraint stress
Mice
0302 clinical medicine
Internal medicine
medicine
Neurites
Premovement neuronal activity
Animals
Psychiatry
Serotonin receptor
5-HT receptor
Neurons
business.industry
Depression
Plant Extracts
General Medicine
lcsh:Other systems of medicine
lcsh:RZ201-999
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
Complementary and alternative medicine
Receptor, Serotonin, 5-HT1A
Receptor, Serotonin, 5-HT1B
Serotonin
business
030217 neurology & neurosurgery
Stress, Psychological
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- BMC Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....4821514df059784fbd7d7e6049d65b2a