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The Clathrin Adaptor AP-1A Mediates Basolateral Polarity
- Source :
- Developmental Cell. (4):811-823
- Publisher :
- Elsevier Inc.
-
Abstract
- SummaryClathrin and the epithelial-specific clathrin adaptor AP-1B mediate basolateral trafficking in epithelia. However, several epithelia lack AP-1B, and mice knocked out for AP-1B are viable, suggesting the existence of additional mechanisms that control basolateral polarity. Here, we demonstrate a distinct role of the ubiquitous clathrin adaptor AP-1A in basolateral protein sorting. Knockdown of AP-1A causes missorting of basolateral proteins in MDCK cells, but only after knockdown of AP-1B, suggesting that AP-1B can compensate for lack of AP-1A. AP-1A localizes predominantly to the TGN, and its knockdown promotes spillover of basolateral proteins into common recycling endosomes, the site of function of AP-1B, suggesting complementary roles of both adaptors in basolateral sorting. Yeast two-hybrid assays detect interactions between the basolateral signal of transferrin receptor and the medium subunits of both AP-1A and AP-1B. The basolateral sorting function of AP-1A reported here establishes AP-1 as a major regulator of epithelial polarity.
- Subjects :
- Endosome
Adaptor Protein Complex 1
Fluorescent Antibody Technique
Endosomes
Biology
medicine.disease_cause
Clathrin
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
symbols.namesake
0302 clinical medicine
Dogs
Two-Hybrid System Techniques
Protein targeting
Cell polarity
Receptors, Transferrin
medicine
Animals
RNA, Small Interfering
Molecular Biology
Cells, Cultured
030304 developmental biology
Epithelial polarity
0303 health sciences
Cell Membrane
Cell Polarity
Epithelial Cells
Cell Biology
Golgi apparatus
Transport protein
Cell biology
Protein Transport
Receptors, LDL
symbols
biology.protein
Clathrin adaptor proteins
030217 neurology & neurosurgery
Developmental Biology
trans-Golgi Network
Subjects
Details
- Language :
- English
- ISSN :
- 15345807
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Developmental Cell
- Accession number :
- edsair.doi.dedup.....4819adf9bcf5a01fe96402910b06b5ce
- Full Text :
- https://doi.org/10.1016/j.devcel.2012.02.004