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Hypoxia Attenuates PGE2but Increases Prostacyclin and Thromboxane Production in Human Term Villous Trophoblast
- Source :
- Placenta. 22:519-525
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- Prostanoids have been proposed to play a major role in the regulation of uteroplacental blood flow. We examined the effect of hypoxia on the production of prostaglandin E(2)(PGE(2)) thromboxane B(2)(TXB(2)), and prostacyclin (measured as 6-keto-PGF(1alpha)) by human term trophoblast cells and villous placental explants. Explants (n=8) and purified trophoblast cells (n=5) were incubated for 24-72 h under either normoxic (21 per cent O(2)) or hypoxic (2 per cent O(2)) conditions. In trophoblast monolayer cultures, hypoxia attentuated PGE(2)production rates to 52+/-9.4 per cent (mean+/-sem, P0.05) but recovered to control rates within 48 h. In villous explants, PGE(2)production was significantly decreased after 48 and 72 h of hypoxia versus the normoxic control, accompanied by increased production of 6-keto-PGF(1alpha)to 173.9+/-26.7 per cent after 48 h. TXB(2)production was increased to 172.3+/-25.9 per cent and 653.2+/-135.7 per cent (P0.05) control after 48 and 72 h of hypoxia, respectively. These results were confirmed in villous explants (n=3) cultured in the presence of exogenous 10 microm arachidonic acid. Hypoxia had no significant effect on TXB(2)and 6-keto-PGF(1alpha)in trophoblast cells. In summary, our findings suggest that hypoxia could be responsible for abnormal profiles of prostanoid production commonly observed in women with pre-eclampsia. These results indicate a putative link between hypoxia and compromised placental perfusion.
- Subjects :
- medicine.medical_specialty
Placenta
Prostacyclin
6-Ketoprostaglandin F1 alpha
Biology
Dinoprostone
Thromboxane Production
Thromboxane A2
chemistry.chemical_compound
Pregnancy
Internal medicine
medicine
Humans
Prostaglandin E2
Cells, Cultured
Obstetrics and Gynecology
Prostanoid
Trophoblast
Hypoxia (medical)
Cell Hypoxia
Trophoblasts
Oxygen
Thromboxane B2
Kinetics
medicine.anatomical_structure
Endocrinology
Reproductive Medicine
chemistry
Eicosanoid
Female
lipids (amino acids, peptides, and proteins)
medicine.symptom
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 01434004
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Placenta
- Accession number :
- edsair.doi.dedup.....4806e029b6f7c32d92ce40de8b607270