Microbiome and oral squamous cell carcinoma: a possible interplay on iron metabolism and its impact on tumor microenvironment

Made available in DSpace on 2021-06-25T15:05:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-05-18 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) There is increasing evidence showing positive association between changes in oral microbiome and the occurrence of oral squamous cell carcinoma (OSCC). Alcohol- and nicotine-related products can induce microbial changes but are still unknown if these changes are related to cancerous lesion sites. In an attempt to understand how these changes can influence the OSCC development and maintenance, the aim of this study was to investigate the oral microbiome linked with OSCC as well as to identify functional signatures and associate them with healthy or precancerous and cancerous sites. Our group used data of oral microbiomes available in public repositories. The analysis included data of oral microbiomes from electronic cigarette users, alcohol consumers, and precancerous and OSCC samples. An R-based pipeline was used for taxonomic and functional prediction analysis. The Streptococcus spp. genus was the main class identified in the healthy group. Haemophilus spp. predominated in precancerous lesions. OSCC samples revealed a higher relative abundance compared with the other groups, represented by an increased proportion of Fusobacterium spp., Prevotella spp., Haemophilus spp., and Campylobacter spp. Venn diagram analysis showed 52 genera exclusive of OSCC samples. Both precancerous and OSCC samples seemed to present a specific associated functional pattern. They were menaquinone-dependent protoporphyrinogen oxidase pattern enhanced in the former and both 3 ',5 '-cyclic-nucleotide phosphodiesterase (purine metabolism) and iron(III) transport system ATP-binding protein enhanced in the latter. We conclude that although precancerous and OSCC samples present some differences on microbial profile, both microbiomes act as iron chelators-like potentially contributing to tumor growth. Univ Fed Rio Grande do Sul, Fac Dent, Prevent & Community Dent Dept, BR-90035003 Porto Alegre, RS, Brazil Univ Sao Paulo, Fac Med Ribeirao Preto, Postgrad Program Clin Oncol Stem Cells & Cell The, BR-14049900 Ribeirao Preto, SP, Brazil Reg Blood Ctr Ribeirao Preto, Natl Inst Sci & Technol Stem Cell & Cell Therapy, Ctr Cell Based Therapy CEPID FAPESP, Mol Genet & Bioinfonnat Lab MGBL, Rua Tenente Catao Roxo 2501, BR-14049900 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Ribeirio Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP, Brazil Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049900 Ribeirao Preto, SP, Brazil Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo, SP, Brazil Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, BR-13563120 Sao Carlos, SP, Brazil Inst Canc Res IPEC, BR-85015430 Guarapuava, PR, Brazil Univ Estadual Centro Oeste, Dept Med, BR-85015430 Guarapuava, PR, Brazil Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Food & Nutr, Araraquara, SP, Brazil Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto, SP, Brazil Univ Paulista, Inst Hlth Sci, Undergrad Biol Sci, Ribeirao Preto, SP, Brazil Univ Fed Parana, Grad Program Bioinformat Profess & Technol Educ S, Curitiba, Parana, Brazil Univ Fed Amapa, Dept Biol Sci & Hlth, Macapa, AP, Brazil Embrapa Southeast Livestock EMBRAPA, Sao Paulo, Brazil FARVET, Labs Invest & Desarrollo, Chincha Alta, Ica, Peru Univ San Martin de Porres, Ctr Invest Genet & Biol Mol CIGBM, Lima, Peru Para State Univ, Grad Program Parasit Biol Amazon, Belem, Para, Brazil Para State Univ, Inst Technol, Dept Elect & Biomed Engn, Belem, Para, Brazil Fundacao Ctr Controle Oncol Estado Amazonas, Dept Educ & Res, Manaus, Amazonas, Brazil Univ Fed Goias, Inst Pathol Trop & Publ Hlth, Goiania, Go, Brazil Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral Maxillofacial Surg & Periodontol, Ribeirao Preto, SP, Brazil Univ Sao Paulo, Sch Arts Sci & Humanities, Sao Paulo, Brazil Blood Ctr Ribeirao Preto, Ctr Cell Based Therapy CEPID FAPESP, Mol Genet & Bioinformat Lab MGBL, Rua Tenente Catao Roxo 2501, BR-14051140 Ribeirao Preto, SP, Brazil Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Food & Nutr, Araraquara, SP, Brazil CNPq: 465539/2014-9 FAPESP: 2013/08135-2 FAPESP: 17/23205-8 FAPESP: 19/08528-0