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Transfection of antisense oligodeoxynucleotide inhibits heparanase gene expression and invasive ability of human pancreatic cancer cellin vitro
- Source :
- Journal of Huazhong University of Science and Technology [Medical Sciences]. 26:72-74
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Extracellular matrix (ECM) degradation is an essential step that allows tumor cells to penetrate a tissue barrier and become metastatic. Heparanase (HPSE) is an endoglycosidase that specifically degrades heparin sulfate proteoglycans (HSPG), a chief component of ECM. HPSE is not expressed in normal epithelial cells but can be detected in a variety of human carcinomas including pancreatic cancer. In the present study, human pancreatic cancer cell line Panc-1 was transfected with HPSE antisense oligodeoxynucleotide (AS-ODN) in vitro, then the inhibitory effect of AS-ODN on HPSE gene expression and invasive ability of Panc-1 cells in vitro was examined. The HPSE mRNA and protein expression of Panc-1 cells transfected with AS-ODN was significantly inhibited. However, there were no marked inhibitory effects in Panc-1 cells treated with nonsense oligodeoxynucleotide (NS-ODN). Moreover, a modified Boyden chamber assay demonstrated that transfection with HPSE AS-ODN significantly inhibited invasive potential of Panc-1 cells in vitro after AS-ODN transfection. This suggests that HPSE AS-ODN may contribute to the inhibition of HPSE mRNA and protein expression, and results in a decrease of the invasive ability of Panc-1 in vitro.
- Subjects :
- endocrine system diseases
Biomedical Engineering
Biology
Biochemistry
Biomaterials
Extracellular matrix
Cell Line, Tumor
Pancreatic cancer
Gene expression
Genetics
medicine
Humans
Neoplasm Invasiveness
Heparanase
RNA, Messenger
Glucuronidase
Earth-Surface Processes
Messenger RNA
hemic and immune systems
Transfection
Oligonucleotides, Antisense
respiratory system
medicine.disease
Molecular biology
In vitro
Extracellular Matrix
Pancreatic Neoplasms
Cell culture
Cancer research
Subjects
Details
- ISSN :
- 19931352 and 16720733
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Huazhong University of Science and Technology [Medical Sciences]
- Accession number :
- edsair.doi.dedup.....47ed6faf92e064f0375d68af4634f042