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Improved detection of non-tuberculous mycobacteria in hospital water samples
- Source :
- Infectious Diseases Now. 51:488-491
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Non-tuberculous mycobacteria (NTM) are opportunistic pathogens commonly colonizing hospital water systems, and may be responsible for healthcare-associated infections (HAI). Investigation of HAI and outbreaks caused by NTM necessitates water analyses. However, NTM are slow-growing bacteria within the mesophilic community present in water, and are difficult to detect. Prior to culture on specific media, their recovery usually requires decontamination and concentration steps. We assessed the effectiveness of filtration as regards the recovery of 7 NTM species in hospital water samples. We also compared the use of cetylpyridinium chloride (CPC) at different concentrations and Sodium Hydroxide (NaOH) 4% in decontamination of water samples with mesophilic bacteria. Our laboratory protocol showed that membrane filtration was suitable for concentration and recovery of NTM from water. Sample decontamination with CPC was more NTM-preservative than NaOH. A combination of CPC at 0.005% and filtration allowed detection of NTM at low concentrations, ranging from 3 to 98 CFU/100mL according to the NTM species.
- Subjects :
- Healthcare associated infections
Veterinary medicine
biology
Chemistry
Water
Cetylpyridinium
Nontuberculous Mycobacteria
Human decontamination
Water safety
bacterial infections and mycoses
Cetylpyridinium chloride
biology.organism_classification
Hospitals
Culture Media
law.invention
chemistry.chemical_compound
Infectious Diseases
law
Filtration
Bacteria
Volume concentration
Mesophile
Subjects
Details
- ISSN :
- 26669919
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Infectious Diseases Now
- Accession number :
- edsair.doi.dedup.....47de3fc22725a9fb16a4d7949df338a5