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CD26/dipeptidyl peptidase IV differentially regulates the chemotaxis of T cells and monocytes toward RANTES: possible mechanism for the switch from innate to acquired immune response
- Source :
- International Immunology. 11:417-426
- Publication Year :
- 1999
- Publisher :
- Oxford University Press (OUP), 1999.
-
Abstract
- CD26, a 110 kDa cell surface glycoprotein, exhibits dipeptidyl peptidase IV (DPPIV; EC 3.4.14.5) enzyme activity and plays an important role in T cell co-stimulation. In the present study, the function of CD26/DPPIV in transendothelial migration was examined using beta-chemokines as chemoattractants. When soluble recombinant CD26 (sCD26/DPPIV+) was added to the transendothelial chemotaxis system, chemotactic migration of T cells toward RANTES was significantly enhanced. Addition of sCD26 to 50 ng/ml of RANTES enhanced the migratory response by a factor of two compared to RANTES alone, whereas mutant soluble CD26 (mCD26), lacking the DPPIV enzyme activity, had no enhancing effect on RANTES-induced T cell migration. In the process of analyzing the mechanisms of the enhancement of T cell migration by sCD26, we showed that RANTES was cleaved by sCD26 under physiologic conditions at the precise site characteristic of its enzyme specificity. However, synthesized RANTES which lacks two N-terminal amino acids showed a chemotactic activity equivalent to full-length RANTES on T cells. Furthermore, addition of sCD26 showed enhancement of T cell migration induced by both forms of RANTES. In contrast to T cells, the truncated RANTES is inactive in chemotaxis of purified monocytes and supplement of sCD26 but not mCD26 reduced the migratory response of monocytes to RANTES. These results suggest that CD26/DPPIV differentially regulate the chemotactic response of T cells and monocytes to RANTES.
- Subjects :
- Dipeptidyl Peptidase 4
T-Lymphocytes
T cell
Molecular Sequence Data
Immunology
Cell
Biology
Monocytes
CCL5
Dipeptidyl peptidase
medicine
Humans
Immunology and Allergy
Drug Interactions
Amino Acid Sequence
Chemokine CCL5
Dipeptidyl peptidase-4
Chemotaxis
General Medicine
T lymphocyte
Molecular biology
Peptide Fragments
Chemotaxis, Leukocyte
medicine.anatomical_structure
Solubility
T cell migration
Endothelium, Vascular
Subjects
Details
- ISSN :
- 14602377 and 09538178
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- International Immunology
- Accession number :
- edsair.doi.dedup.....47d55d9eda2d0adb21c35dac72d86196