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Antiviral activity of gliotoxin, gentian violet and brilliant green against Nipah and Hendra virus in vitro
- Source :
- Virology Journal, Vol 6, Iss 1, p 187 (2009), Virology Journal
- Publication Year :
- 2009
-
Abstract
- Background Using a recently described monolayer assay amenable to high throughput screening format for the identification of potential Nipah virus and Hendra virus antivirals, we have partially screened a low molecular weight compound library (>8,000 compounds) directly against live virus infection and identified twenty eight promising lead molecules. Initial single blind screens were conducted with 10 μM compound in triplicate with a minimum efficacy of 90% required for lead selection. Lead compounds were then further characterised to determine the median efficacy (IC50), cytotoxicity (CC50) and the in vitro therapeutic index in live virus and pseudotype assay formats. Results While a number of leads were identified, the current work describes three commercially available compounds: brilliant green, gentian violet and gliotoxin, identified as having potent antiviral activity against Nipah and Hendra virus. Similar efficacy was observed against pseudotyped Nipah and Hendra virus, vesicular stomatitis virus and human parainfluenza virus type 3 while only gliotoxin inhibited an influenza A virus suggesting a non-specific, broad spectrum activity for this compound. Conclusion All three of these compounds have been used previously for various aspects of anti-bacterial and anti-fungal therapy and the current results suggest that while unsuitable for internal administration, they may be amenable to topical antiviral applications, or as disinfectants and provide excellent positive controls for future studies.
- Subjects :
- viruses
Drug Evaluation, Preclinical
Cercopithecus aethiop
Genome, Viral
medicine.disease_cause
Antiviral Agents
lcsh:Infectious and parasitic diseases
Microbiology
Hendra Virus
03 medical and health sciences
chemistry.chemical_compound
Gliotoxin
Quaternary Ammonium Compound
Virology
parasitic diseases
Chlorocebus aethiops
Influenza A virus
medicine
Animals
lcsh:RC109-216
Cytotoxicity
Hendra Viru
Vero Cells
030304 developmental biology
Antiviral Agent
0303 health sciences
biology
Molecular Structure
030306 microbiology
Animal
Research
Nipah Virus
virus diseases
biology.organism_classification
In vitro
3. Good health
Quaternary Ammonium Compounds
Human Parainfluenza Virus
Nipah Viru
Infectious Diseases
chemistry
Vesicular stomatitis virus
Vero cell
Vero Cell
Gentian Violet
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Virology Journal, Vol 6, Iss 1, p 187 (2009), Virology Journal
- Accession number :
- edsair.doi.dedup.....47c4bd972be5890540e61865573448a9