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Assessing physio-macromolecular effects of lactic acid on Zygosaccharomyces bailii cells during microaerobic fermentation
- Source :
- FEMS yeast research. 16(5)
- Publication Year :
- 2016
-
Abstract
- The ability of Zygosaccharomyces bailii to grow at low pH and in the presence of considerable amounts of weak organic acids, at lethal condition for Saccharomyces cerevisiae , increased the interest in the biotechnological potential of the yeast. To understand the mechanism of tolerance and growth effect of weak acids on Z. bailii , we evaluated the physiological and macromolecular changes of the yeast exposed to sub lethal concentrations of lactic acid. Lactic acid represents one of the important commodity chemical which can be produced by microbial fermentation. We assessed physiological effect of lactic acid by bioreactor fermentation using synthetic media at low pH in the presence of lactic acid. Samples collected from bioreactors were stained with propidium iodide (PI) which revealed that, despite lactic acid negatively influence the growth rate, the number of PI positive cells is similar to that of the control. Moreover, we have performed Fourier Transform Infra-Red (FTIR) microspectroscopy analysis on intact cells of the same samples. This technique has been never applied before to study Z. bailii under this condition. The analyses revealed lactic acid induced macromolecular changes in the overall cellular protein secondary structures, and alterations of cell wall and membrane physico-chemical properties.
- Subjects :
- 0301 basic medicine
Chemical Phenomena
Protein Conformation
Zygosaccharomyces bailii
Saccharomyces cerevisiae
Bioreactor
Zygosaccharomyces
Microbiology
Applied Microbiology and Biotechnology
Cell wall
Fungal Proteins
03 medical and health sciences
chemistry.chemical_compound
Bioreactors
Cell Wall
Stress, Physiological
Spectroscopy, Fourier Transform Infrared
Propidium iodide
Anaerobiosis
Lactic Acid
Microbial Viability
biology
Staining and Labeling
Medicine (all)
Cell Membrane
food and beverages
General Medicine
Hydrogen-Ion Concentration
biology.organism_classification
CHIM/11 - CHIMICA E BIOTECNOLOGIA DELLE FERMENTAZIONI
Yeast
Lactic acid
Culture Media
030104 developmental biology
chemistry
Biochemistry
FTIR
Fermentation
Propidium
Subjects
Details
- ISSN :
- 15671364
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- FEMS yeast research
- Accession number :
- edsair.doi.dedup.....47b7b09b0d0367f722c355eab39f8fa5