Back to Search
Start Over
Cytokines and inflammatory mediators: Markers involved in interstitial damage to the pancreas in two dengue fever cases associated with acute pancreatitis
- Source :
- PLoS ONE, Vol 17, Iss 1, p e0262785 (2022), PLoS ONE
- Publication Year :
- 2022
- Publisher :
- Public Library of Science (PLoS), 2022.
-
Abstract
- Dengue viral (DENV) infections can lead to acute pancreatitis and associated tissue damage. This study examined the pancreas from two fatal cases of DENV for histopathological changes as well as for the detection of cytokines, and other inflammatory mediators. Tissue sections were prepared for examination by ultrastructural and histopathological techniques. Sections from the pancreas of non-infected individuals were prepared in parallel as a control. The presence of viral replication in macrophages was detected by co-staining for the proteins NS3 and CD68 by immunofluorescence. Immunohistochemistry was used to detect cells that expressed cytokines and inflammatory mediators to characterize the inflammatory response. Edema, acinar necrosis and fibrosis areas associated with a mononuclear infiltrate were found in infected tissues. The major site of virus replication appeared to be macrophages based on their exclusive presentation of the viral protein NS3. Pancreatic tissues from the infected individuals also displayed increased levels of high mobility group box-1, caspase-3, gelatinase B and tumor necrosis factor alpha compared to controls. The presence of virus replicating macrophages in the pancreas was associated with multiple changes in tissue structure that included elevated levels of cytokines and inflammatory markers that may differentiate acute pancreatitis due to DENV infections from other causes.
- Subjects :
- Male
RNA viruses
Viral Diseases
Physiology
viruses
Apoptosis
Pathology and Laboratory Medicine
Biochemistry
Dengue Fever
Dengue
White Blood Cells
Medical Conditions
Animal Cells
Immune Physiology
Medicine and Health Sciences
Innate Immune System
Multidisciplinary
Middle Aged
Infectious Diseases
Medical Microbiology
Viral Pathogens
Viruses
Cytokines
Medicine
Female
Inflammation Mediators
Pathogens
Cellular Types
Anatomy
Research Article
Neglected Tropical Diseases
Adult
Immune Cells
Science
Immunology
Endocrine System
Gastroenterology and Hepatology
Microbiology
Young Adult
Exocrine Glands
Humans
Microbial Pathogens
Pancreas
Blood Cells
Flaviviruses
Macrophages
Organisms
Biology and Life Sciences
Proteins
Cell Biology
Dengue Virus
Molecular Development
Tropical Diseases
Fibrosis
Pancreatitis
Immune System
Collagens
Biomarkers
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....47b241c924c4e1d5363bfe8ccba602c6