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Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2014
- Publisher :
- MyJove Corporation, 2014.
-
Abstract
- Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.
- Subjects :
- Adventitia
Pathology
medicine.medical_specialty
Stromal cell
General Immunology and Microbiology
Cluster of differentiation
General Chemical Engineering
General Neuroscience
Mesenchymal stem cell
Endothelial Cells
Skeletal muscle
Mesenchymal Stem Cells
Biology
Cell sorting
General Biochemistry, Genetics and Molecular Biology
Cellular Biology
medicine.anatomical_structure
Precursor cell
medicine
Humans
Pericyte
Stem cell
Muscle, Skeletal
Pericytes
Tunica Intima
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....479643f87678d73982cb35d0881a908e