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Photodynamic therapy using 5-aminolaevulinic acid for experimental pancreatic cancer--prolonged animal survival
- Source :
- British Journal of Cancer
- Publication Year :
- 1994
-
Abstract
- Experimental studies have been carried out using 5-aminolaevulinic acid (ALA) to induce transient porphyrin photosensitisation for photodynamic therapy (PDT) in a pancreatic cancer model in Syrian golden hamsters. ALA was given either intravenously or orally (in bolus or fractionated doses) with the laser light delivered by means of a bare fibre touching the tissue surface or external irradiation using a light-integrating cylindrical applicator. Animals were killed 1-24 h after ALA administration for pharmacokinetic studies and 3-7 days after light exposure to study PDT-induced necrosis. A separate survival study was also performed after a fractionated oral dose of ALA and external irradiation. Protoporphyrin IX sensitisation in the tumour tissue as measured by quantitative fluorescence microscopy was highest after intravenous administration of 200 mg kg-1 ALA and then in decreasing order after oral fractionated and oral bolus doses (both 400 mg kg-1). Laser light application at 630 nm to give 12-50 J from the bare fibre or 50 J cm-2 using surface illumination with the cylindrical applicator resulted in tumour necrosis up to 8 mm in depth. In larger tumours a rim of viable tumour was observed on the side opposite to illumination. In a randomised study, survival of treated animals was significantly longer than in the untreated control group (log-rank test, P < 0.02), although all animals died of recurrent tumour. This technique shows promise in the treatment of small volumes of tumour in the pancreas. Images Figure 2 Figure 3 Figure 4
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
Necrosis
Pancreatic disease
Time Factors
medicine.medical_treatment
Administration, Oral
Protoporphyrins
Photodynamic therapy
Heme
Pharmacology
Sensitivity and Specificity
chemistry.chemical_compound
Pharmacokinetics
Cricetinae
medicine
Animals
Protoporphyrin IX
Mesocricetus
business.industry
Lasers
Aminolevulinic Acid
medicine.disease
Pancreatic Neoplasms
Disease Models, Animal
medicine.anatomical_structure
Oncology
chemistry
Microscopy, Fluorescence
Photochemotherapy
Injections, Intravenous
Protoporphyrin
Female
medicine.symptom
business
Pancreas
Bolus (radiation therapy)
Neoplasm Transplantation
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 15321827 and 00070920
- Volume :
- 70
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....478d9facf3139947e303fcb0ec9157c6