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Novel Poxvirus Infection in an Immune Suppressed Patient
- Source :
- Clinical Infectious Diseases. 61:1543-1548
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- BACKGROUND Human and animal poxvirus infections are being reported with increasing frequency. We describe a challenging case history and treatment of a previously unknown poxvirus rash illness in a renal transplant patient. METHODS A combination of classical microbiology techniques, including viral culture and electron microscopy, were used to provide initial clinical diagnosis. Subsequent standard polymerase chain reaction assays available in 2001 were noncontributory. Next generation sequencing was used to provide definitive diagnosis. RESULTS Retrospectively, next generation sequencing methods were used to ultimately provide the definitive diagnosis of a novel poxvirus infection initially identified by electron microscopy. The closest relative of this poxvirus, identified in North America, is a poxvirus collected from a mosquito pool from Central Africa in 1972. CONCLUSIONS This diagnostic quandary was ultimately solved using next generation DNA sequencing. This article describes the use of classical and next generation diagnostic strategies to identify etiologic agents of emerging infectious diseases and once again demonstrates the susceptibility of immunossupressed patients to novel pathogens. The virus identified is closely related to Yoka virus; these viruses appear to have independently diverged from a common ancestor of all known orthopoxviruses.
- Subjects :
- Male
Microbiology (medical)
Virus Cultivation
viruses
Poxviridae Infections
Genome
Virus
DNA sequencing
law.invention
Immunocompromised Host
Microscopy, Electron, Transmission
law
Humans
Poxviridae
Orthopoxvirus
Phylogeny
Polymerase chain reaction
Retrospective Studies
biology
Viral culture
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
Exanthema
Middle Aged
biology.organism_classification
Kidney Transplantation
Virology
Transplant Recipients
Infectious Diseases
Subjects
Details
- ISSN :
- 15376591 and 10584838
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Clinical Infectious Diseases
- Accession number :
- edsair.doi.dedup.....4782e504fb36d4b44fdc4b952277f0f7