Axenic Culture of Anisogramma anomala: Evidence for Self-Inhibition of Ascospore Germination and Colony Growth

A medium supporting the prolonged growth of Anisogramma anomala in axenic culture is described. Ascospores germinated and established colonies only on media containing a chemical adsorbent. Bovine serum albumin at 0.5% and activated charcoal at 0.025% were most effective. Sucrose was the only car? bon source of 19 carbohydrates tested that supported prolonged growth in culture. Growth was further en? hanced by asparagine and yeast extract at 0.2%. Ad- sorbents apparently inactivate self-inhibitors of asco? spore germination as well as growth regulators produced by the growing cultures. Such self-inhibition