Back to Search
Start Over
Identification of five types of forensic body fluids based on stepwise discriminant analysis
- Source :
- Forensic Science International: Genetics. 48:102337
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Peripheral blood, menstrual blood, semen, saliva and vaginal secretions are the five most common body fluids found at crime scenes, and the identification of these five body fluids is of great significance to the reconstruction of a crime scene and resolution of the case. However, accurate identification of these five body fluids is still a challenge. To address this problem, a mathematical model for differentiating five types of forensic body fluids based on the differential expression characteristics of multiple miRNAs in five body fluids (peripheral blood, menstrual blood, semen, saliva and vaginal secretions) was developed. A total of 350 forensic body fluids (70 of each type) were collected and tested, and relative expression of 10 miRNAs (miR-451a, miR-205-5p, miR-203-3p, miR-214-3p, miR-144-3p, miR-144-5p, miR-654-5p, miR-888-5p, miR-891a-5p, miR-124a-3p) in all samples was detected by SYBR Green real-time qPCR. Three hundred samples (60 samples of each body fluid) were used as the training set to screen meaningful identification markers by stepwise discriminant analysis, and a discriminant function was established. Fifty samples (10 samples of each body fluid) were used as a validation set to examine the accuracy of the model, and 25 samples (the types of samples were unknown to the experimenter) were used for a blind test. Except for miR-144-3p, the other miRNAs were selected to construct discriminant analysis models. The self-validation accuracy of the model was 99.7 %, cross-validation accuracy was 99.3 %, accuracy of the identification validation set was 100 %, and accuracy of the blind test result was 100 %. This study provides a reliable and accurate identification strategy for five common body fluids (peripheral blood, menstrual blood, semen, saliva, and vaginal secretions) in forensic medicine.
- Subjects :
- Adult
Forensic Genetics
Male
0301 basic medicine
Saliva
Physiology
Semen
Real-Time Polymerase Chain Reaction
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Discriminant function analysis
Genetics
Humans
Medicine
030216 legal & forensic medicine
Body fluid
business.industry
Stepwise discriminant analysis
Discriminant Analysis
Linear discriminant analysis
Menstruation
Forensic science
MicroRNAs
Blood
030104 developmental biology
Cervix Mucus
Female
Identification (biology)
business
Biomarkers
Subjects
Details
- ISSN :
- 18724973
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Forensic Science International: Genetics
- Accession number :
- edsair.doi.dedup.....4769b4f6e2d77ffdb7696cd1bdaf47d1