Back to Search
Start Over
Cafestol Inhibits Cyclic-Strain-Induced Interleukin-8, Intercellular Adhesion Molecule-1, and Monocyte Chemoattractant Protein-1 Production in Vascular Endothelial Cells
- Source :
- Oxidative Medicine and Cellular Longevity, Vol 2018 (2018), Oxidative Medicine and Cellular Longevity
- Publication Year :
- 2018
- Publisher :
- Hindawi Limited, 2018.
-
Abstract
- Moderate coffee consumption is inversely associated with cardiovascular disease mortality; however, mechanisms underlying this causal effect remain unclear. Cafestol, a diterpene found in coffee, has various properties, including an anti-inflammatory property. This study investigated the effect of cafestol on cyclic-strain-induced inflammatory molecule secretion in vascular endothelial cells. Cells were cultured under static or cyclic strain conditions, and the secretion of inflammatory molecules was determined using enzyme-linked immunosorbent assay. The effects of cafestol on mitogen-activated protein kinases (MAPK), heme oxygenase-1 (HO-1), and sirtuin 1 (Sirt1) signaling pathways were examined using Western blotting and specific inhibitors. Cafestol attenuated cyclic-strain-stimulated intercellular adhesion molecule-1 (ICAM-1), monocyte chemoattractant protein- (MCP-) 1, and interleukin- (IL-) 8 secretion. Cafestol inhibited the cyclic-strain-induced phosphorylation of extracellular signal-regulated kinase and p38 MAPK. By contrast, cafestol upregulated cyclic-strain-induced HO-1 and Sirt1 expression. The addition of zinc protoporphyrin IX, sirtinol, or Sirt1 silencing (transfected with Sirt1 siRNA) significantly attenuated cafestol-mediated modulatory effects on cyclic-strain-stimulated ICAM-1, MCP-1, and IL-8 secretion. This is the first study to report that cafestol inhibited cyclic-strain-induced inflammatory molecule secretion, possibly through the activation of HO-1 and Sirt1 in endothelial cells. The results provide valuable insights into molecular pathways that may contribute to the effects of cafestol.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Aging
Article Subject
p38 mitogen-activated protein kinases
Intercellular Adhesion Molecule-1
Cafestol
Biochemistry
p38 Mitogen-Activated Protein Kinases
03 medical and health sciences
Sirtuin 1
Stress, Physiological
medicine
Human Umbilical Vein Endothelial Cells
Humans
Interleukin 8
Phosphorylation
RNA, Small Interfering
lcsh:QH573-671
Extracellular Signal-Regulated MAP Kinases
Chemokine CCL2
Kinase
Chemistry
lcsh:Cytology
Interleukin-8
Interleukin
Cell Biology
General Medicine
Cell biology
Up-Regulation
030104 developmental biology
RNA Interference
Signal transduction
Diterpenes
Reactive Oxygen Species
Corrigendum
Heme Oxygenase-1
Research Article
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 19420994 and 19420900
- Volume :
- 2018
- Database :
- OpenAIRE
- Journal :
- Oxidative Medicine and Cellular Longevity
- Accession number :
- edsair.doi.dedup.....475633105a25a390f877c5681322cbaf