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Histidine-rich peptides and polymers for nucleic acids delivery
- Source :
- Advanced Drug Delivery Reviews, Advanced Drug Delivery Reviews, Elsevier, 2001, 53 (1), pp.75-94
- Publication Year :
- 2001
- Publisher :
- HAL CCSD, 2001.
-
Abstract
- International audience; Nucleic acids transfer into mammalian cells requires devices to improve their escape from endocytic vesicles where they are mainly confined following cellular uptake. In this review, we describe histidine-rich molecules that enable the transfer of plasmid and oligonucleotides (ODN) in human and non-human cultured cells. An histidine-rich peptide which permeabilizes biological membrane at pH 6.4, favored the transfection mediated by lactosylated polylysine/pDNA complexes. Histidylated polylysine forms cationic particles of 100 nm with a plasmid and yielded a transfection of 3-4.5 orders of magnitude higher than polylysine. The biological activity of antisense ODN was increased more than 20-fold when it was complexed with highly histidylated oligolysine into small cationic spherical particles of 35 nm. Evidence that imidazole protonation mediates the effect of these molecules in endosomes are provided. We also describe a disulfide-containing polylysine conjugate capable of mediating DNA unpackaging in a reductive medium and to increase the transfection efficiency. Overall, these molecules constitute interesting devices for developing non-viral gene delivery systems.
- Subjects :
- Endosome
Polymers
[SDV]Life Sciences [q-bio]
Pharmaceutical Science
Peptide
02 engineering and technology
Biology
Gene delivery
03 medical and health sciences
chemistry.chemical_compound
Nucleic Acids
Animals
Humans
Histidine
Cells, Cultured
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Oligonucleotide
Gene Transfer Techniques
Transfection
021001 nanoscience & nanotechnology
[SDV] Life Sciences [q-bio]
Endocytic vesicle
Biochemistry
chemistry
Polylysine
Nucleic acid
0210 nano-technology
Peptides
Subjects
Details
- Language :
- English
- ISSN :
- 0169409X
- Database :
- OpenAIRE
- Journal :
- Advanced Drug Delivery Reviews, Advanced Drug Delivery Reviews, Elsevier, 2001, 53 (1), pp.75-94
- Accession number :
- edsair.doi.dedup.....475307d73d658e3c451c6bbda6b26dd1