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Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes
- Source :
- Molecular Biology of the Cell, Molecular Biology of the Cell, American Society for Cell Biology, 1997, 8 (2), pp.249-261. ⟨10.1091/mbc.8.2.249⟩, ResearcherID
- Publication Year :
- 1997
- Publisher :
- American Society for Cell Biology (ASCB), 1997.
-
Abstract
- International audience; The G2 arrest of oocytes from frogs, clams, and starfish requires that preformed cyclin B-cdc2 complexes [prematuration-promoting factor (MPF)] be kept in an inactive form that is largely due to inhibitory phosphorylation of this pre-MPF. We have investigated the role of mitogen-activated protein (MAP) kinase in the activation of this pre-MPF. The cytoplasm of both frog and starfish oocytes contains an activity that can rapidly inactivate injected MPF. When the MAP kinase of G2-arrested starfish or Xenopus oocytes was prematurely activated by microinjection of c-mos or Ste-11 delta N fusion proteins, the rate and extent of MPF inactivation was much reduced. Both effects were suppressed by expression of the specific MAP kinase phosphatase Pyst 1. These results show that MAP kinase down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in Xenopus oocytes. In starfish oocytes, however, MAP kinase activation occurs only after germinal vesicle breakdown, much after MPF activation. In this case, down-regulation of the cyclin B-cdc2 inhibiting pathway is a sensitive response to hormonal stimulation that does not require MAP kinase activation.
- Subjects :
- G2 Phase
Xenopus
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Down-Regulation
Mitogen-activated protein kinase kinase
Biology
MAP2K7
Starfish
03 medical and health sciences
0302 clinical medicine
Cyclins
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Amino Acid Sequence
Molecular Biology
reproductive and urinary physiology
030304 developmental biology
Cell Nucleus
0303 health sciences
MAP kinase kinase kinase
urogenital system
Cyclin-dependent kinase 2
Cyclin-dependent kinase 3
Cell Biology
Molecular biology
Hormones
Rats
Enzyme Activation
Calcium-Calmodulin-Dependent Protein Kinases
embryonic structures
Oocytes
Cyclin-dependent kinase complex
MAP kinase phosphatase
biology.protein
Female
Cyclin-dependent kinase 7
CDC28 Protein Kinase, S cerevisiae
030217 neurology & neurosurgery
Research Article
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....4752a50eae10072299caef46f38e3f01