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Enteral but not parenteral antibiotics enhance gut function and prevent necrotizing enterocolitis in formula-fed newborn preterm pigs
- Source :
- Birck, M M, Nguyen, D N, Cilieborg, M S, Kamal, S, S. Nielsen, D, Damborg, P, Olsen, J E, Lauridsen, C, Sangild, P T & Thymann, T 2016, ' Enteral but not parenteral antibiotics enhance gut function and prevent necrotizing enterocollitis in forumula-fed newborn preterm pigs ', American Journal of Physiology: Gastrointestinal and Liver Physiology, vol. 310, no. 5, pp. G323-G333 . https://doi.org/10.1152/ajpgi.00392.2015
- Publication Year :
- 2015
-
Abstract
- Preterm infants are susceptible to infection and necrotizing enterocolitis (NEC) and are often treated with antibiotics. Simultaneous administration of enteral and parenteral antibiotics during the first days after preterm birth prevents formula-induced NEC lesions in pigs, but it is unknown which administration route is most effective. We hypothesized that only enteral antibiotics suppress gut bacterial colonization and NEC progression in formula-fed preterm pigs. Caesarean-delivered preterm pigs (90–92% of gestation) were fed increasing amounts of infant formula from birth to day 5 and given saline (CON) or antibiotics (ampicillin, gentamicin, and metronidazole) via the enteral (ENT) or parenteral (PAR) route ( n = 16–17). NEC lesions, intestinal morphology, function, microbiology, and inflammatory mediators were evaluated. NEC lesions were completely prevented in ENT pigs, whereas there were high incidences of mild NEC lesions (59–63%) in CON and PAR pigs ( P < 0.001). ENT pigs had elevated intestinal weight, villus height/crypt depth ratio, and goblet cell density and reduced gut permeability, mucosal adherence of bacteria, IL-8 levels, colonic lactic acid levels, and density of Gram-positive bacteria, relative to CON pigs ( P < 0.05). Values in PAR pigs were intermediate with few affected parameters (reduced lactic acid levels and density and adherence of Gram-positive bacteria, relative to CON pigs, P < 0.05). There was no evidence of increased antimicrobial resistance following the treatments. We conclude that enteral, but not parenteral, administration of antibiotics reduces gut bacterial colonization, inflammation, and NEC lesions in newborn, formula-fed preterm pigs. Delayed colonization may support intestinal structure, function, and immunity in the immediate postnatal period of formula-fed preterm neonates.
- Subjects :
- 0301 basic medicine
Physiology
Swine
Antibiotics
Enteral administration
Gastroenterology
antibiotics
0302 clinical medicine
Pregnancy
Ampicillin
Intubation, Gastrointestinal
Infant Formula
Anti-Bacterial Agents
Intestines
Treatment Outcome
Premature birth
Necrotizing enterocolitis
Premature Birth
enteral antibiotics
030211 gastroenterology & hepatology
Gentamicin
Female
medicine.drug
medicine.medical_specialty
medicine.drug_class
Biological Availability
Biology
Gram-Positive Bacteria
03 medical and health sciences
Enterocolitis, Necrotizing
Physiology (medical)
Internal medicine
Metronidazole
medicine
Animals
Infusions, Intra-Arterial
necrotizing enterocolitis
Hepatology
medicine.disease
digestive system diseases
Surgery
Gastrointestinal Microbiome
Disease Models, Animal
030104 developmental biology
Infant formula
Animals, Newborn
preterm pig
Gentamicins
Subjects
Details
- ISSN :
- 15221547
- Volume :
- 310
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Accession number :
- edsair.doi.dedup.....4749d0abca8390286b930e953319b38e