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Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber
- Source :
- Optics letters, vol 44, iss 9, Opt Lett
- Publication Year :
- 2019
- Publisher :
- The Optical Society, 2019.
-
Abstract
- Fiber-based imaging of tissue autofluorescence using ultraviolet (UV) excitation is a highly flexible tool used to probe structure and composition. In this Letter, we report, to the best of our knowledge, the first results from a single-fiber imaging system employing a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two distinct spatial resolutions. We characterize the lateral point spread function and fluorescent background of the system and show how enhanced resolution can identify features such as trabeculae in ex vivo murine bone samples.
- Subjects :
- Point spread function
Fluorescence-lifetime imaging microscopy
Materials science
Multispectral image
Bioengineering
Optical Physics
02 engineering and technology
01 natural sciences
Article
010309 optics
Optics
Double-clad fiber
0103 physical sciences
Fiber
Electrical and Electronic Engineering
Image resolution
Quantum Physics
business.industry
021001 nanoscience & nanotechnology
Fluorescence
Atomic and Molecular Physics, and Optics
Good Health and Well Being
0210 nano-technology
business
Photonic-crystal fiber
Subjects
Details
- ISSN :
- 15394794 and 01469592
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Optics Letters
- Accession number :
- edsair.doi.dedup.....46fc15d8dc77c73ab4b7e1d7524fa997