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Simultaneous determination of nine coumarins in rat plasma by HPLC–MS/MS for pharmacokinetics studies following oral administration of Fraxini Cortex extract
- Source :
- Journal of Chromatography B. 1025:25-32
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the simultaneous determination of nine coumarins including aesculin, aesculetin, fraxin, fraxetin, scopoletin, isoscopoletin, 6-hydroxy-7,8-dimethoxy coumarin, 8-hydroxy-6,7-dimethoxy coumarin and umbelliferone in rat plasma using nodakenin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with methanol. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of methanol and water (containing 0.05% acetic acid). All analytes and IS were quantitated through electrospray ionization in negative ion multiple reaction monitoring (MRM) mode. This method was fully validated in terms of the sensitivity, specificity, accuracy, precision (intra- and inter-day), matrix effect, recovery as well as the stability of the analyte under various conditions, and the results satisfied the requirements of biological sample measurement. The validated method was successfully applied to pharmacokinetic study of the nine coumarins in rat plasma after oral administration of Fraxini Cortex aqueous extract, among which the pharmacokinetics of four coumarins including fraxetin, isoscopoletin, 6-hydroxy-7,8-dimethoxy coumarin and 8-hydroxy-6,7-dimethoxy coumarin were studied for the first time.
- Subjects :
- Male
Clinical Biochemistry
Administration, Oral
Aesculus
Umbelliferone
Sensitivity and Specificity
030226 pharmacology & pharmacy
01 natural sciences
Biochemistry
Analytical Chemistry
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Coumarins
Tandem Mass Spectrometry
Scopoletin
Animals
Protein precipitation
heterocyclic compounds
Chromatography, High Pressure Liquid
Chromatography
Chemistry
010401 analytical chemistry
Selected reaction monitoring
Reproducibility of Results
Cell Biology
General Medicine
Coumarin
Rats
0104 chemical sciences
Linear Models
Fraxin
Fraxetin
Aesculetin
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 1025
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....46f57ef96f5fbbaa17f4bbd2d60216ec