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Increased Expression of Costimulatory Molecules on Peripheral Blood Monocytes in Patients with Crohn's Disease
- Source :
- Scandinavian Journal of Gastroenterology. 32:1241-1246
- Publication Year :
- 1997
- Publisher :
- Informa UK Limited, 1997.
-
Abstract
- Activation of T lymphocytes and monocytes/macrophages has been implicated in the pathogenesis of Crohn's disease (CD). Costimulatory molecules play important roles in optimal T-cell activation.With flow cytometric analysis we have investigated the expression of the costimulatory molecules B7-1 (CD80), B7-2 (CD86), and CD18 and the intercellular adhesion molecule-1 (ICAM-1) on peripheral blood monocytes and the expression of the activation markers HLA-DR and IL-2R (CD25) on peripheral blood T lymphocytes from 31 CD patients, 17 ulcerative colitis (UC) patients, and 10 healthy controls.In CD patients the percentage of activated T cells (CD3+ HLA-DR+ and CD3+ IL-2R+) was significantly increased compared with those of controls and UC patients (P0.05). Most monocytes from all three groups expressed B7-2, CD18, and ICAM-1 molecules (all79%), but only a few positive cells expressed B7-1 molecules (5%). No significant differences were detected in the percentage positivity of all costimulatory molecules tested among CD, UC, and controls. The mean fluorescence intensity (MFI) of B7-1 in all three groups was very weak and not significantly different. However, in CD patients there was a significantly increased MFI of B7-2, CD18, and ICAM-1 molecules compared with UC and controls (P0.05). On the other hand, both the percentage positivity and MFI of HLA-DR molecules on monocytes of UC patients were significantly lower than those of CD patients and controls (P0.05).Expression of the costimulatory molecules B7-2, CD18, and ICAM-1 on peripheral blood monocytes of CD patients is increased. In CD patients activation of peripheral T lymphocytes may correlate with increased expression of these costimulatory molecules on peripheral blood monocytes.
- Subjects :
- Adult
Antigens, Differentiation, T-Lymphocyte
Male
Adolescent
T-Lymphocytes
CD3
Monocytes
Pathogenesis
Crohn Disease
Antigens, CD
medicine
HLA-DR
Humans
IL-2 receptor
CD86
Membrane Glycoproteins
biology
business.industry
Monocyte
Gastroenterology
Receptors, Interleukin-2
HLA-DR Antigens
T lymphocyte
Middle Aged
Flow Cytometry
Intercellular Adhesion Molecule-1
medicine.anatomical_structure
Fluorescent Antibody Technique, Direct
CD18 Antigens
Immunology
B7-1 Antigen
biology.protein
Colitis, Ulcerative
Female
B7-2 Antigen
business
CD80
Subjects
Details
- ISSN :
- 15027708 and 00365521
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Scandinavian Journal of Gastroenterology
- Accession number :
- edsair.doi.dedup.....46ea8ea628d3e479f424a3bf6cc11ae3