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DNA damage in human T-lymphoblastoid cell line Molt-3 induced by reactive oxygen species
- Source :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 285:225-228
- Publication Year :
- 1993
- Publisher :
- Elsevier BV, 1993.
-
Abstract
- In the course of studying the effect of reactive oxygen species such as superoxide anion (O 2 − ), hydrogen peroxide (H 2 O 2 ) on oxidant-sensitive human T-lymphoblastoid cell line Molt-3, O 2 − has been generated by the interaction of xanthine with xanthine oxidase (XOD). To confirm that H 2 O 2 is a key intermediate for inducing DNA single-strand breaks in Molt-3 cells, studies have been carried out with pure H 2 O 2 . In the presence of xanthine + XOD or H 2 O 2 the amount of DNA single-strand breaks has been found found to increase as a function of the O 2 − and H 2 O 2 concentration. Data from studies with antioxidants such as superoxide dismutase and catalase supports a mechanism of DNA damage dependent on the presence of H 2 O 2 in Molt-3 cells. Molt-3 cells are CD4 + and sensitive to reactive oxygen stress and therefore, could be an ideal cell line for determining the relationship between oxidative stress and various diseases such as acquired immunodeficiency syndrome (AIDS).
- Subjects :
- CD4-Positive T-Lymphocytes
Xanthine Oxidase
DNA damage
Health, Toxicology and Mutagenesis
medicine.disease_cause
Cell Line
Superoxide dismutase
chemistry.chemical_compound
Genetics
medicine
Humans
Xanthine oxidase
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
biology
Superoxide Dismutase
Chemistry
Superoxide
Hydrogen Peroxide
Catalase
Xanthine
Biochemistry
biology.protein
Reactive Oxygen Species
Oxidative stress
DNA Damage
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 285
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- Accession number :
- edsair.doi.dedup.....46cffaf75fb72715c0958958740e5151