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Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity
Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity
- Source :
- Journal of virology. 69(8)
- Publication Year :
- 1995
-
Abstract
- Previously, we demonstrated induction of a unique macrophage prothrombinase during infection of BALB/cJ mice by mouse hepatitis virus strain 3 (MHV-3). By immunologic screening, a clone representing this prothrombinase was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp but, when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrated when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
- Subjects :
- Immunology
Molecular Sequence Data
Clone (cell biology)
Biology
Transfection
Microbiology
Virus
Thromboplastin
Mice
Mouse hepatitis virus
Prothrombinase
Virology
Complementary DNA
Animals
Amino Acid Sequence
RNA, Messenger
Peptide sequence
Cells, Cultured
Mice, Inbred BALB C
Murine hepatitis virus
Base Sequence
cDNA library
Fibrinogen
biology.organism_classification
Blotting, Northern
Molecular biology
FGL2
Insect Science
Enzyme Induction
DNA, Viral
Research Article
Subjects
Details
- ISSN :
- 0022538X
- Volume :
- 69
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of virology
- Accession number :
- edsair.doi.dedup.....46c72b1474386d3cd3b7ebc26ac1326b