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Exploring the capacity of aquatic biofilms to act as environmental DNA samplers: Test on macroinvertebrate communities in rivers
- Source :
- Science of the Total Environment, Science of the Total Environment, Elsevier, 2021, 763, pp.144208. ⟨10.1016/j.scitotenv.2020.144208⟩
- Publication Year :
- 2021
- Publisher :
- HAL CCSD, 2021.
-
Abstract
- International audience; Aquatic biofilms are heterogeneous assemblages of microorganisms surrounded by a matrix of extracellular polymeric substances (EPS). Recent studies suggest that aquatic biofilms can physically act as sorptive sponges of DNA. We took the opportunity from already available samples of stone biofilms and macroinvertebrates specimens collected in parallel at the same sites to test the capacity of biofilms to act as DNA samplers of macroinvertebrate communities in streams. Macroinvertebrate communities are usually studied with metabarcoding using the DNA extracted from their bodies bulk samples, which remains a time-consuming approach and involves the destruction of all individual specimens from the samples. The ability of biofilms to capture DNA was explored on 19 rivers sites of a tropical island (Mayotte Island, France). First, macroinvertebrate specimens were identified based on their morphological characteristics. Second, DNA was extracted from biofilms, and macroinvertebrate communities were targeted using a standard COI barcode. The resulting morphological and molecular inventories were compared. They provided comparable structures and diversities for macroinvertebrate communities when one is working with the unassigned OTU data. After taxonomic assignment of the OTU data, diversity and richness were no longer correlated. The ecological assessment derived from morphological bulk samples was conserved by the biofilms samples. We also showed that the biofilm method allows to detect a higher diversity for some organisms (Cnidaria), that is hardly accessible with the morphological method. The results of this study exploring the DNA signal captured by natural biofilms are encouraging. However, a more detailed study integrating more replicates and comparing the biodiversity signal based on both morphological and molecular bulk macroinvertebrate samples to the one captured by biofilms will be necessary. Better understanding how the DNA signal captured by natural biofilms represents the biodiversity of a given sampling site is necessary before considering its use for bioassessment applications.
- Subjects :
- Environmental Engineering
010504 meteorology & atmospheric sciences
Microorganism
Biodiversity
010501 environmental sciences
01 natural sciences
Comoros
Extracellular polymeric substance
Rivers
Environmental Chemistry
Animals
Environmental DNA
14. Life underwater
Waste Management and Disposal
Ecosystem
Biofilms Metabarcoding Environmental DNA Macroinvertebrates
0105 earth and related environmental sciences
Invertebrate
Ecology
Biofilm
Ecological assessment
DNA
biochemical phenomena, metabolism, and nutrition
Pollution
DNA, Environmental
Invertebrates
Biofilms
[SDE]Environmental Sciences
Environmental science
Species richness
France
Environmental Monitoring
Subjects
Details
- Language :
- English
- ISSN :
- 00489697 and 18791026
- Database :
- OpenAIRE
- Journal :
- Science of the Total Environment, Science of the Total Environment, Elsevier, 2021, 763, pp.144208. ⟨10.1016/j.scitotenv.2020.144208⟩
- Accession number :
- edsair.doi.dedup.....46bcbaec0ffd2221a2112740cb7537f8