Additional file 1 of Engineering a HEK-293T exosome-based delivery platform for efficient tumor-targeting chemotherapy/internal irradiation combination therapy

Additional file 1: Fig. S1. (A) UV–Vis spectra of the Dox, iRGD-Exos, and Dox@iRGD-Exos from 250 to 700 nm. (B) Standard concentration curve of Dox constructed with measurements made by a UV-3600 plus UV–Vis spectrophotometer. Fig. S2. Radiochemical efficiency and purity of 131I-labeled Dox@iRGD-Exos. (A) Radiochemical efficiency and (B) purity were measured by instant thin-layer chromatography (TLC) with an AR-2000 radio-TLC imaging scanner. Fig. S3. Stability of the as-prepared exosomes. The changes in diameter of (A) blank-Exos and (B) Dox@iRGD-Exos-131I at 4 °C in 1 × PBS and at 37 °C in serum, respectively, over 7 days. Data are shown as the mean ± SD (n = 3). Fig. S4. Cell viability assay. Viability of (A) 8505C and (B) Hth7 cells treated with different concentrations of Dox or Dox@iRGD-Exos for 24 h. A CCK-8 assay was used to assess cell viability in each group. NS, not significant; * indicates P