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Galactose Oxidase/Prussian Blue Based Biosensors
- Source :
- Electroanalysis, Electroanalysis, Wiley-VCH Verlag, 2015, 27, pp.1341-1344. ⟨10.1002/elan.201400720⟩, Electroanalysis, 2015, 27, pp.1341-1344. ⟨10.1002/elan.201400720⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- International audience; This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-(O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag-AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx-TK biosensor.
- Subjects :
- LDH
Inorganic chemistry
02 engineering and technology
Amperometric biosensor
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
Electrochemistry
[CHIM]Chemical Sciences
Hydrogen peroxide
Prussian blue
010401 analytical chemistry
021001 nanoscience & nanotechnology
Amperometry
0104 chemical sciences
chemistry
Prussian Blue
Galactose oxidase
Screen-printed electrode
Electrode
Transketolase activity
Clays
Transketolase
0210 nano-technology
Biosensor
Subjects
Details
- Language :
- English
- ISSN :
- 10400397 and 15214109
- Database :
- OpenAIRE
- Journal :
- Electroanalysis, Electroanalysis, Wiley-VCH Verlag, 2015, 27, pp.1341-1344. ⟨10.1002/elan.201400720⟩, Electroanalysis, 2015, 27, pp.1341-1344. ⟨10.1002/elan.201400720⟩
- Accession number :
- edsair.doi.dedup.....46aac834e32e361ec9141b25c5bdc4f9