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Increased oxidative phosphorylation in lymphocytes does not atone for decreased cell numbers after burn injury
- Source :
- Innate Immunity, Innate Immunity, Vol 26 (2020)
- Publication Year :
- 2020
- Publisher :
- SAGE Publications, 2020.
-
Abstract
- The acute systemic inflammatory response syndrome (SIRS) and multiorgan dysfunction (MOD) that occur in large burn injuries may be attributed, in part, to immunosuppressive responses such as decreased lymphocytes. However, the mitochondrial bioenergetics of lymphocytes after severe burn injury are poorly understood. The purpose of this study was to examine mitochondrial function of lymphocytes following severe burns in a swine model. Anesthetized Yorkshire swine ( n = 17) sustained 40% total body surface area full-thickness contact burns. Blood was collected at pre-injury (Baseline; BL) and at 24 and 48 h after injury for complete blood cell analysis, flow cytometry, cytokine analysis, and ficoll separation of intact lymphocytes for high-resolution mitochondrial respirometry analysis. While neutrophil numbers increased, a concomitant decrease was found in lymphocytes ( P + or CD8+ lymphocytes. No changes in immune cell population were observed from 24 h to 48 h post-injury. IL 12-23 decreased while a transient increase in IL 4 was found from BL to 24h ( P
- Subjects :
- lcsh:Immunologic diseases. Allergy
CD4-Positive T-Lymphocytes
0301 basic medicine
Burn injury
Swine
Lymphocyte
Cell Respiration
Immunology
Cell
oxidative phosphorylation
Cell Count
Burn
Oxidative phosphorylation
CD8-Positive T-Lymphocytes
lymphocyte
Mitochondrion
Microbiology
03 medical and health sciences
0302 clinical medicine
Animals
Humans
Medicine
Lymphocytes
Molecular Biology
Cells, Cultured
business.industry
030208 emergency & critical care medicine
Multiorgan dysfunction
Original Articles
Cell Biology
medicine.disease
Mitochondria
Systemic inflammatory response syndrome
Disease Models, Animal
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Cytokines
lcsh:RC581-607
Burns
business
Subjects
Details
- ISSN :
- 17534267 and 17534259
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Innate Immunity
- Accession number :
- edsair.doi.dedup.....46933cf41e38fa9ca66d67ff21c9b9f4