Additional file 1: of Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients

Additional methods. Table S1. High-resolution HLA typing for HLA-A, HLA-B, HLA-C, HLA-DRB1, and HLA-DQB1 of MSCs and PBMCs utilized in this study. Figure S1. Promotion effects of iPSC-MSCs on resting PBMCs were not only due to allogeneic recognition. Figure S2. Activation of CD4+ and CD8+ T cells in both AR patients’ and normal PBMCs with stimulation of PMA and ionomycin. Figure S3. Representative gating strategies for CD4+ and CD8+ T cells in this study. Figure S4. Representative gating strategies for Treg cells in this study. Figure S5. Foxp3 mRNA expression in PBMCs from normal and AR patients after coculture with MSCs. Figure S6. IL-10 and IFN-γ levels secreted by iPSC-MSCs/PBMCs under different conditions. Figure S7. PGE2 levels in PBMCs from healthy control and AR patients when cocultured with MSCs and NS398. Figure S8. ICAM-1, VCAM-1, and PD-1 expression on iPSC-MSCs when cocultured with PBMCs (DOCX 14612 kb)