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Metabolic Phenotyping Using UPLC–MS and Rapid Microbore UPLC–IM–MS: Determination of the Effect of Different Dietary Regimes on the Urinary Metabolome of the Rat
- Source :
- Chromatographia. 83:853-861
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- A rapid reversed-phase gradient method employing a 50 mm × 1 mm i.d., C18 microbore column, combined with ion mobility and high-resolution mass spectrometry, was applied to the metabolic phenotyping of urine samples obtained from rats receiving different diets. This method was directly compared to a “conventional” method employing a 150 × 2.1 mm i.d. column packed with the same C18 bonded phase using the same samples. Multivariate statistical analysis of the resulting data showed similar class discrimination for both microbore and conventional methods, despite the detection of fewer mass/retention time features by the former. Multivariate statistical analysis highlighted a number of ions that represented diet-specific markers in the samples. Several of these were then identified using the combination of mass, ion-mobility-derived collision cross section and retention time including N-acetylglutamate, urocanic acid, and xanthurenic acid. Kynurenic acid was tentatively identified based on mass and ion mobility data.
- Subjects :
- Biochemistry & Molecular Biology
Metabolic phenotyping
MOBILITY-MASS-SPECTROMETRY
PREDICTION
Ion mobility
Clinical Biochemistry
Urine
Mass spectrometry
Proteomics
01 natural sciences
Biochemistry
High-performance liquid chromatography
Biochemical Research Methods
Analytical Chemistry
Microbore columns
03 medical and health sciences
chemistry.chemical_compound
Kynurenic acid
0399 Other Chemical Sciences
Metabolome
Xanthurenic acid
030304 developmental biology
0303 health sciences
Science & Technology
Chromatography
Chemistry, Analytical
010401 analytical chemistry
Organic Chemistry
Metabolite identification
HPLC-MS
0104 chemical sciences
Chemistry
Urocanic acid
chemistry
Physical Sciences
Life Sciences & Biomedicine
0301 Analytical Chemistry
Subjects
Details
- ISSN :
- 16121112 and 00095893
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- Chromatographia
- Accession number :
- edsair.doi.dedup.....4682e80d70aa952cbd5ce420798c9ea9