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Metabolic Phenotyping Using UPLC–MS and Rapid Microbore UPLC–IM–MS: Determination of the Effect of Different Dietary Regimes on the Urinary Metabolome of the Rat

Authors :
Nyasha Munjoma
Susan E. Slade
Robert S. Plumb
J. Swann
Jeremy K. Nicholson
Ian D. Wilson
Marine P.M. Letertre
Muireann Coen
Source :
Chromatographia. 83:853-861
Publication Year :
2020
Publisher :
Springer Science and Business Media LLC, 2020.

Abstract

A rapid reversed-phase gradient method employing a 50 mm × 1 mm i.d., C18 microbore column, combined with ion mobility and high-resolution mass spectrometry, was applied to the metabolic phenotyping of urine samples obtained from rats receiving different diets. This method was directly compared to a “conventional” method employing a 150 × 2.1 mm i.d. column packed with the same C18 bonded phase using the same samples. Multivariate statistical analysis of the resulting data showed similar class discrimination for both microbore and conventional methods, despite the detection of fewer mass/retention time features by the former. Multivariate statistical analysis highlighted a number of ions that represented diet-specific markers in the samples. Several of these were then identified using the combination of mass, ion-mobility-derived collision cross section and retention time including N-acetylglutamate, urocanic acid, and xanthurenic acid. Kynurenic acid was tentatively identified based on mass and ion mobility data.

Details

ISSN :
16121112 and 00095893
Volume :
83
Database :
OpenAIRE
Journal :
Chromatographia
Accession number :
edsair.doi.dedup.....4682e80d70aa952cbd5ce420798c9ea9