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Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds
- Source :
- PLoS ONE, PLOS ONE, 13 (12), Article: e0207397, PLoS ONE, Vol 13, Iss 12, p e0207397 (2018)
- Publication Year :
- 2018
- Publisher :
- Public Library of Science, 2018.
-
Abstract
- As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required. We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells. Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized. Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity. Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks. This was more pronounced in Coll and hybrid gels than in pure HA gels. Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli. Fibroblast cell culture proved the biocompatibility of all investigated compositions. Coll containing gels enabled cell migration, adhesion and proliferation inside the gels.
- Subjects :
- Life sciences
biology
Polymers
Cell Survival
Science
Amorphous Solids
Materials Science
Immunology
Biocompatible Materials
Research and Analysis Methods
Animal Cells
ddc:570
Medicine and Health Sciences
Humans
Cell Cycle and Cell Division
Hyaluronic Acid
Materials
Connective Tissue Cells
Cell Proliferation
Tissue Engineering
Tissue Scaffolds
Viscosity
Biology and Life Sciences
Cell Biology
Cell Cultures
Fibroblasts
Polymer Chemistry
Elasticity
Extracellular Matrix
Biomechanical Phenomena
Chemistry
Kinetics
Biological Tissue
Macromolecules
Connective Tissue
Cell Processes
Mixtures
Physical Sciences
Medicine
Feasibility Studies
Biocompatibility
Biological Cultures
Collagen
Cellular Types
Anatomy
Cellular Structures and Organelles
Gels
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....46710f83920ff0728f63b5aafd949d3b