Back to Search
Start Over
Glycosylation-related genes are variably expressed depending on the differentiation state of a bioaminergic neuronal cell line: implication for the cellular prion protein
- Source :
- Glycoconjugate Journal, Glycoconjugate Journal, Springer Verlag, 2009, 26 (4), pp.477-493. ⟨10.1007/s10719-008-9198-5⟩, Glycoconjugate Journal, 2009, 26 (4), pp.477-493. ⟨10.1007/s10719-008-9198-5⟩
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- Chantier qualité GA; A striking feature of the cellular prion protein (PrPC) is the heterogeneity of its glycoforms, whose contribution to PrPC function has yet to be defined. Using the 1C11 neuronal bioaminergic differentiation model and a glycomics approach, we show here a correlation between differential PrPC N-glycosylations in 1C115-HT serotonergic and 1C11NE noradrenergic cells compared to their 1C11 precursor cells and a variation of the glycogenome expression status in these cells. In particular, expression of genes involved in N-glycan synthesis or in the modeling of chondroitin and heparan sulfate proteoglycans appeared to be modulated. Our results highlight that, the expression of glycosylation-related genes is regulated during bioaminergic neuronal differentiation, consistent with a participation of glycoconjugates in neuronal development and plasticity. A neuronal regulation of glycosylation processes may have direct implications on some neurospecific functions of PrPC and may participate in specific brain targeting of prion strains.
- Subjects :
- Electrophoresis
Biogenic Amines
Glycosylation
Prions
Glycoconjugate
animal diseases
[SDV]Life Sciences [q-bio]
Enzyme-Linked Immunosorbent Assay
Biology
Biochemistry
Cell Line
Glycomics
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
N-linked glycosylation
Precursor cell
glycotranscriptome
n-glycosylation
neuronal differentiation
Molecular Biology
Gene
Phylogeny
glycoconjugate
Glycosaminoglycans
030304 developmental biology
Neurons
chemistry.chemical_classification
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Stem Cells
Reproducibility of Results
Cell Differentiation
Cell Biology
nervous system diseases
Cell biology
carbohydrates (lipids)
Gene Expression Regulation
chemistry
Cell culture
prpc
030217 neurology & neurosurgery
Function (biology)
Subjects
Details
- ISSN :
- 15734986 and 02820080
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Glycoconjugate Journal
- Accession number :
- edsair.doi.dedup.....4657627611a94df9f5d2cc1dbcc94204