Back to Search
Start Over
Maternal glyphosate-based herbicide exposure alters antioxidant-related genes in the brain and serum metabolites of male rat offspring
- Source :
- Neurotoxicology. 74
- Publication Year :
- 2018
-
Abstract
- In response to the rapid development of genetically engineered glyphosate-tolerant crops, the use of glyphosate-based herbicides (GBHs), in agriculture, has increased substantially. Currently, it is estimated that 747 million kg of GBHs are applied per year. Although several epidemiological studies have demonstrated that there are health risks associated with GBH exposure, the effects these chemicals have on the oxidative and inflammatory response in the brain are still unclear. In fact, alterations in these processes could contribute to the development of neurological diseases, such as Alzheimer’s disease and autism spectrum disorders. The present study exposed pregnant rats to GBH and evaluated changes in the expression of genes related to oxidnte defense and inflammation response and monitored the serum metabolome in the adult male offspring. Pregnant Wistar rats were administered distilled water or Roundup®, at either 5 and 50 mg/kg/day, (p.o.) from gestational day (GD) 18 to postnatal day (PND) 5. There was a significant increase in the gene expression levels of Neuroglobin (Ngb – oxygen storage and tissue protection) (105%, p = 0.031), Glutathione Peroxidase 1 (Gpx1 – oxidative stress) (95%, p = 0.005), Prostaglandin-Endoperoxidase Synthase 1 (Ptgs1 - inflammation) (109%, p = 0.033) and Hypoxia inducible factor 1 subunit alpha (Hif1α – oxygen sensor) (73%, p = 0.017), in the cerebellum of PND90 rats perinatally exposed to 50 mg GBH/kg/day. Moreover, both GBH-exposed groups displayed a significant decrease in the expression of Catalase (Cat – oxidative stress) (49%, p = 0.003; and 31% p = 0.050, respectively) expression, in the cortex. Serum metabolites analyses, from the same animals of each group, demonstrated that there were significant changes in the concentrations of lysophosphatidylcholine and phosphatidylcholine, which have been associated with neurodegenerative diseases. The results of the present study suggest GBH exposure during pregnancy alters the expression of genes associated with oxidant defense, inflammation and lipid metabolism. It is plausible that maternal GBH exposure could have lasting neuronal effects on the offspring later in life.
- Subjects :
- Male
medicine.medical_specialty
GPX1
Antioxidant
Offspring
medicine.medical_treatment
Glycine
Alpha (ethology)
Gestational Age
Biology
Toxicology
medicine.disease_cause
Antioxidants
03 medical and health sciences
0302 clinical medicine
Pregnancy
Internal medicine
medicine
Metabolome
Animals
Gene Regulatory Networks
Rats, Wistar
030304 developmental biology
Brain Chemistry
Inflammation
0303 health sciences
Herbicides
General Neuroscience
Lipid metabolism
Lipid Metabolism
Rats
Oxidative Stress
Endocrinology
Gene Expression Regulation
Catalase
Maternal Exposure
Prenatal Exposure Delayed Effects
biology.protein
Female
030217 neurology & neurosurgery
Oxidative stress
Subjects
Details
- ISSN :
- 18729711
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Neurotoxicology
- Accession number :
- edsair.doi.dedup.....464c3ec4221ef6221942ecf3185ea7ef