Back to Search
Start Over
Immunohistochemical evaluation of infiltrating immune cells in endometrial biopsy of female genital tuberculosis
- Source :
- European journal of obstetrics, gynecology, and reproductive biology. 267
- Publication Year :
- 2021
-
Abstract
- Background Female Genital Tuberculosis (FGTB) causes infertility in a large number of females in developing countries. Presence of granuloma on histopathological examination of endometrial samples is diagnostic of FGTB. But immunohistochemical evaluation of endometrial aspirates has not been explored before. Aim To evaluate the immunohistochemical delineation of immune cells in FGTB. Methods 1515 infertile women from 20 to 35 years were enrolled and underwent endometrial aspiration (EA), which was subjected to microbiological and histopathological examination along with PCR. Patients positive for conventional tests like granulomas, acid fast bacilli, mycobacterial culture on LJ medium or liquid (MGIT) culture were started on antitubercular therapy. Conventional test negative but PCR positive patients were posted for laparoscopy. Immunohistochemistry (IHC) for LCA, CD68, CD3, CD4, CD8, CD 20, CD138, IFN gamma and IL10 were evaluated. Result 38/1515 (2.5%) subjects tested positive for conventional methods. PCR-TB was positive in 615/1515 samples (40.59%). On IHC, the number of CD45 (LCA) positive immune cells (p = 0.03) and IFN gamma (p = 0.002) and IL10 expression (p = 0.012) at 1 + level were higher in the PCR positive samples. Laparoscopy done in 418/463 patients and 89/418 (21.3%) showed definitive findings of tuberculosis. CD3, CD4, CD8, CD20, CD68 and CD138 showed no correlation with PCR and laparoscopy. Conclusion Increased IFN gamma and IL 10 expressing immune cells in PCR positive EA suggests subclinical early changes, and can be useful as a research tool but have no role in diagnosing FGTB.
- Subjects :
- Pathology
medicine.medical_specialty
Tuberculosis
Biopsy
Antitubercular Agents
Endometrium
Immune system
medicine
Humans
Subclinical infection
CD20
medicine.diagnostic_test
biology
business.industry
Obstetrics and Gynecology
Mycobacterium tuberculosis
medicine.disease
Tuberculosis, Female Genital
medicine.anatomical_structure
Reproductive Medicine
Granuloma
biology.protein
Immunohistochemistry
Female
business
Infertility, Female
Endometrial biopsy
Subjects
Details
- ISSN :
- 18727654
- Volume :
- 267
- Database :
- OpenAIRE
- Journal :
- European journal of obstetrics, gynecology, and reproductive biology
- Accession number :
- edsair.doi.dedup.....4638c8ccf485a356819e3dce353437db