New conditions for matrix-assisted laser desorption/ionization mass spectrometry of native bacterial R-type lipopolysaccharides

Authors :: Luisa Sturiale
Domenico Garozzo
Alba Silipo
Antonio Molinaro
Rosa Lanzetta
Michelangelo Parrilli
L., Sturiale
D., Garozzo
Silipo, Alba
Lanzetta, Rosa
Parrilli, Michelangelo
Molinaro, Antonio
Source :: RCM. Rapid communications in mass spectrometry 19 (2005): 1829–1834. doi:10.1002/rcm.1994, info:cnr-pdr/source/autori:L.Sturiale, D.Garozzo, A.Silipo, R.Lanzetta, M.Parrilli, A.Molinaro/titolo:New conditions for matrix-assisted laser desorption%2Fionization mass spectrometry of native bacterial R-type lipopolysaccharides/doi:10.1002%2Frcm.1994/rivista:RCM. Rapid communications in mass spectrometry/anno:2005/pagina_da:1829/pagina_a:1834/intervallo_pagine:1829–1834/volume:19
Publication Year :: 2005
Publisher :: Wiley, 2005.
Abstract: A new sample preparation method for matrix-assisted laser desorption/ionization (MALDI) analysis of native rough-type lipopolysaccharides (R-type LPSs) is presented. In our MALDI mass spectra, besides the [MH]− ions, abundant ions originating from the cleavage between the 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) unit and the lipid A moiety are always present, giving important pieces of information about the structure of the molecules analyzed. Remarkably, in most cases, the comparison of the MALDI mass spectra of the intact R-type LPS with the O-deacylated one allowed us to obtain the structure of the lipid A moiety. Copyright © 2005 John Wiley & Sons, Ltd.

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