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Rabies virus matrix protein interplay with eIF3, new insights into rabies virus pathogenesis
- Source :
- Nucleic Acids Research, Nucleic Acids Research, Oxford University Press, 2007, 35 (5), pp.1522-32. ⟨10.1093/nar/gkl1127⟩, Nucleic acids research, vol 35, iss 5, Nucleic Acids Research, 2007, 35 (5), pp.1522-32. ⟨10.1093/nar/gkl1127⟩
- Publication Year :
- 2007
-
Abstract
- Viral proteins are frequently multifunctional to accommodate the high density of information encoded in viral genomes. Matrix (M) protein of negative-stranded RNA viruses such as Rhabdoviridae is one such example. Its primary function is virus assembly/budding but it is also involved in the switch from viral transcription to replication and the concomitant down regulation of host gene expression. In this study we undertook a search for potential rabies virus (RV) M protein's cellular partners. In a yeast two-hybrid screen the eIF3h subunit was identified as an M-interacting cellular factor, and the interaction was validated by co-immunoprecipitation and surface plasmon resonance assays. Upon expression in mammalian cell cultures, RV M protein was localized in early small ribosomal subunit fractions. Further, M protein added in trans inhibited in vitro translation on mRNA encompassing classical (Kozak-like) 5'-UTRs. Interestingly, translation of hepatitis C virus IRES-containing mRNA, which recruits eIF3 via a different noncanonical mechanism, was unaffected. Together, the data suggest that, as a complement to its functions in virus assembly/budding and regulation of viral transcription, RV M protein plays a role in inhibiting translation in virus-infected cells through a protein-protein interaction with the cellular translation machinery.
- Subjects :
- MESH: Eukaryotic Initiation Factor-3
viruses
Eukaryotic Initiation Factor-3
Messenger
MESH: Amino Acid Sequence
medicine.disease_cause
Viral
0303 health sciences
biology
Biological Sciences
MESH: Protein Subunits
3. Good health
MESH: Rabies virus
MESH: Surface Plasmon Resonance
MESH: Protein Biosynthesis
MESH: RNA, Viral
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
RNA, Viral
MESH: Mutation
Molecular Sequence Data
MESH: Two-Hybrid System Techniques
Virus
Viral Matrix Proteins
03 medical and health sciences
VP40
Viral entry
Information and Computing Sciences
Two-Hybrid System Techniques
Genetics
Viral structural protein
medicine
Immunoprecipitation
Amino Acid Sequence
RNA, Messenger
Molecular Biology
030304 developmental biology
MESH: RNA, Messenger
MESH: Viral Matrix Proteins
Viral matrix protein
MESH: Molecular Sequence Data
030306 microbiology
MESH: Immunoprecipitation
Rabies virus
Rhabdoviridae
Surface Plasmon Resonance
biology.organism_classification
Virology
Internal ribosome entry site
Protein Subunits
Protein Biosynthesis
Mutation
RNA
MESH: Ribosomes
Ribosomes
Environmental Sciences
Developmental Biology
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 35
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Nucleic acids research
- Accession number :
- edsair.doi.dedup.....462705b1a964536c76b678d2ab03920f