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Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance
- Source :
- Cell, Cell, Elsevier, 2000, 101, pp.533-542
- Publication Year :
- 2000
-
Abstract
- Posttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between PTGS, quelling, and RNAi since the Arabidopsis SGS2 protein is similar to an RNA-dependent RNA polymerase like N. crassa QDE-1, controlling quelling, and C. elegans EGO-1, controlling RNAi. In contrast, SGS3 shows no significant similarity with any known or putative protein, thus defining a specific step of PTGS in plants. Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, definitively proving that PTGS is an antiviral defense mechanism that can also target transgene RNA for degradation.
- Subjects :
- 0106 biological sciences
RNA-induced transcriptional silencing
DNA, Plant
RNA-induced silencing complex
Trans-acting siRNA
Molecular Sequence Data
Potyvirus
Arabidopsis
RNA-dependent RNA polymerase
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Genes, Plant
01 natural sciences
Cucumovirus
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Solanum lycopersicum
RNA interference
Gene expression
Gene silencing
Amino Acid Sequence
Gene Silencing
Cloning, Molecular
RNA Processing, Post-Transcriptional
[SDV.BC] Life Sciences [q-bio]/Cellular Biology
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Plant Diseases
Plant Proteins
Genetics
0303 health sciences
biology
Base Sequence
Biochemistry, Genetics and Molecular Biology(all)
Arabidopsis Proteins
fungi
Tobamovirus
Chromosome Mapping
GENETIQUE
biology.organism_classification
RNA-Dependent RNA Polymerase
Mutagenesis
010606 plant biology & botany
Subjects
Details
- ISSN :
- 00928674 and 10974172
- Volume :
- 101
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....46225fd53e3fb73505a874438fada050