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IgE-dependent activation of human mast cells and fMLP-mediated activation of human eosinophils is controlled by the circadian clock
- Source :
- Molecular Immunology. 64:76-81
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Symptoms of allergic attacks frequently exhibit diurnal variations. Accordingly, we could recently demonstrate that mast cells and eosinophils – known as major effector cells of allergic diseases – showed an intact circadian clock. Here, we analyzed the role of the circadian clock in the functionality of mast cells and eosinophils. Human intestinal mast cells (hiMC) were isolated from intestinal mucosa; human eosinophils were isolated from peripheral blood. HiMC and eosinophils were synchronized by dexamethasone before stimulation every 4 h around the circadian cycle by FcɛRI crosslinking or fMLP, respectively. Signaling molecule activation was examined using Western blot, mRNA expression by real-time RT-PCR, and mediator release by multiplex analysis. CXCL8 and CCL2 were expressed and released in a circadian manner by both hiMC and eosinophils in response to activation. Moreover, phosphorylation of ERK1/2, known to be involved in activation of hiMC and eosinophils, showed circadian rhythms in both cell types. Interestingly, all clock genes hPer1 , hPer2 , hCry1 , hBmal1 , and hClock were expressed in a similar circadian pattern in activated and unstimulated cells indicating that the local clock controls hiMC and eosinophils and subsequently allergic reactions but not vice versa .
- Subjects :
- Cell type
Immunology
Circadian clock
CLOCK Proteins
Immunoglobulin E
Dexamethasone
Intestinal mucosa
Circadian Clocks
Humans
Mast Cells
RNA, Messenger
Circadian rhythm
Interleukin 8
Extracellular Signal-Regulated MAP Kinases
Molecular Biology
biology
Cell biology
Enzyme Activation
Eosinophils
Intestines
N-Formylmethionine Leucyl-Phenylalanine
ARNTL
CLOCK
Gene Expression Regulation
biology.protein
Chemokines
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....4616fe255dae61f7683049d336fc1189