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Simultaneous Quantification of DPPG, DEPC and Cholesterol in Propofol Liposome by HPLC-ELSD Using Alkaline Hydrolysis
- Source :
- Journal of Chromatographic Science. 58:53-59
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- A high-performance liquid chromatography method with evaporative light-scattering detection (ELSD) was performed for simultaneous determination of dipalmitoyl phosphatidylglycerol (DPPG), dierucoyl phosphatidylcholine (DEPC) and cholesterol in propofol liposome by the pretreatment of alkaline hydrolysis (temperature, concentration of KOH anhydrous ethanol solution and reaction time were 90°C, 1 mol · L−1 and 10 min, respectively). The analysis was carried out on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with isocratic elution of methanol and 0.1% acetic acid aqueous solution (95:5, v/v) at a flow rate of 1.0 mL · min−1. The column temperature was 30°C. The drift tube temperature of the ELSD system was set at 30°C, and the pressure of carrier gas was 350 KPa. The regression equation revealed a good linear relationship (r = 0.9990–0.9993) during the test ranges. The RSD of stability and repeatability (n = 6) was found to be less than 1.96 and 1.46%, respectively. The average recoveries ranged from 97.90 to 101.00%. The proposed method was validated and showed good precision, stability, repeatability and recovery, which indicated that the method could be readily utilized as a quality evaluation method for the determination of DPPG, DEPC and cholesterol in propofol liposome.
- Subjects :
- 02 engineering and technology
01 natural sciences
High-performance liquid chromatography
Analytical Chemistry
Hydrolysis
chemistry.chemical_compound
Chromatography detector
Propofol
Alkaline hydrolysis
Chromatography, High Pressure Liquid
Liposome
Chromatography
Aqueous solution
010401 analytical chemistry
Temperature
Reproducibility of Results
Phosphatidylglycerols
General Medicine
Repeatability
021001 nanoscience & nanotechnology
0104 chemical sciences
Cholesterol
chemistry
Liposomes
Phosphatidylcholines
Methanol
0210 nano-technology
Subjects
Details
- ISSN :
- 1945239X and 00219665
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatographic Science
- Accession number :
- edsair.doi.dedup.....4604fbf3c6036ffebad70ae1c65adc54