Supplementary Figures from Whole Genomic Copy Number Alterations in Circulating Tumor Cells from Men with Abiraterone or Enzalutamide-Resistant Metastatic Castration-Resistant Prostate Cancer

Figure S1: Reference genomic data in cell lines aCGH. A) Male vs. female. B) Leucocyte vs. T47D cells spiked in blood. C) Male vs. LNCaP. D) ERBB1 and SIX1 amplification in T47D cells spiked into the blood. E) PTEN loss in LNCaP; Figure S2: Data reliability and reproducibility of aCGH experiments. A) a; Sample P36.1 and its replicate, b; Overlap in P36.1 and the P36.1-replicate and c; AR amplification in both independent experiments. B) a; P37.3 and its dye swap, b; Genes comparison in P37.3 vs. P37.3 replicate; Figure S3: CGH array gene view images from selected mCRPC clinical CTCs samples depicting gain in selected regions. A) AR, B) FOXA1, C) CDK12, D) BRD4, E) PXN, F) ABL1 and G) NCOA2; Figure S4: Chromosomal aberration of PI3K family members in patient P32.1 and P32.2 CTCs. A) Copy gains (red) and losses (blue), or copy neutral (white) regions in mCRPC CTCs. B) BTK gain was observed in P32.1 and loss in P32.2 CTCs aCGH analysis.