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Modulation of Exon Skipping and Inclusion by Heterogeneous Nuclear Ribonucleoprotein A1 and Pre-mRNA Splicing Factor SF2/ASF
- Source :
- Molecular and Cellular Biology. 13:2993-3001
- Publication Year :
- 1993
- Publisher :
- Informa UK Limited, 1993.
-
Abstract
- The essential splicing factor SF2/ASF and the heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) modulate alternative splicing in vitro of pre-mRNAs that contain 5' splice sites of comparable strengths competing for a common 3' splice site. Using natural and model pre-mRNAs, we have examined whether the ratio of SF2/ASF to hnRNP A1 also regulates other modes of alternative splicing in vitro. We found that an excess of SF2/ASF effectively prevents inappropriate exon skipping and also influences the selection of mutually exclusive tissue-specific exons in natural beta-tropomyosin pre-mRNA. In contrast, an excess of hnRNP A1 does not cause inappropriate exon skipping in natural constitutively or alternatively spliced pre-mRNAs. Although hnRNP A1 can promote alternative exon skipping, this effect is not universal and is dependent, e.g., on the size of the internal alternative exon and on the strength of the polypyrimidine tract in the preceding intron. With appropriate alternative exons, an excess of SF2/ASF promotes exon inclusion, whereas an excess of hnRNP A1 causes exon skipping. We propose that in some cases the ratio of SF2/ASF to hnRNP A1 may play a role in regulating alternative splicing by exon inclusion or skipping through the antagonistic effects of these proteins on alternative splice site selection.
- Subjects :
- Heterogeneous Nuclear Ribonucleoprotein A1
RNA Splicing
viruses
Tropomyosin
Biology
Heterogeneous ribonucleoprotein particle
environment and public health
Heterogeneous-Nuclear Ribonucleoproteins
Exon
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
RNA Precursors
Animals
Humans
Exonic splicing silencer
Molecular Biology
Ribonucleoprotein
Cell Nucleus
Genetics
Cell-Free System
Serine-Arginine Splicing Factors
Alternative splicing
Nuclear Proteins
RNA-Binding Proteins
Exons
Cell Biology
Recombinant Proteins
Exon skipping
Globins
Ribonucleoproteins
Polypyrimidine tract
Spliceosomes
RNA, Heterogeneous Nuclear
Rabbits
HeLa Cells
Research Article
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....45d682336d5b923d71335cc0c6699e3a